Introduction
Materials and methods
Sample size calculation
Patient and samples
Immunohistochemical staining
Immunohistochemical staining analysis
Statistical analysis
Results
Clinical and pathological features of RB patients
Variable | RB cases (%) | Variable | RB cases (%) |
---|---|---|---|
Age (month, mean ± SD) | 26.37 ± 14.96 | Infiltration of choroid | |
Gender | Yes | 20 (39.22%) | |
Male | 24 (47.06%) | No | 31 (60.78%) |
Female | 27 (52.94%) | Infiltration of optic nerve | |
Laterality | Yes | 28 (54.90%) | |
Unilateral | 49 (96.08%) | No | 23 (45.10%) |
Bilateral | 2 (3.92%) | Differentiation | |
Enucleated eye | Good or moderate | 11 (21.57%) | |
Right | 22 (43.14%) | Poor | 40 (78.43%) |
Left | 29 (56.86%) | Calcification | |
Stage | Yes | 19 (37.25%) | |
Group D | 21 (41.18%) | No | 32 (62.75%) |
Group E | 30 (58.82%) | Necrosis | |
High-risk pathology | Yes | 30 (58.82%) | |
Yes | 33 (64.71%) | No | 21 (41.18%) |
No | 18 (35.29%) |
Lower expression level of NR1D1 and NR2E3 in RB compared to normal retina
Correlation of NR1D1 and NR2E3 expression levels with clinicopathological features of RB patients
Variable | High expression | Low expression | P value |
---|---|---|---|
Age | |||
> 2 years old | 7 | 8 | |
≤ 2 years old | 18 | 18 | |
Total | 25 | 26 | 0.828 |
Gender | |||
Male | 11 | 13 | |
Female | 14 | 13 | |
Total | 25 | 26 | 0.668 |
Laterality | |||
Unilateral | 24 | 25 | |
Bilateral | 1 | 1 | |
Total | 25 | 26 | 0.977 |
Enucleated eye | |||
Right | 10 | 12 | |
Left | 15 | 14 | |
Total | 25 | 26 | 0.657 |
IIRC stage | |||
Group D | 15 | 6 | |
Group E | 10 | 20 | |
Total | 25 | 26 | 0.007* |
High-risk pathology | |||
Yes | 13 | 20 | |
No | 12 | 6 | |
Total | 25 | 26 | 0.063 |
Infiltration of choroid | |||
Yes | 6 | 14 | |
No | 19 | 12 | |
Total | 25 | 26 | 0.029* |
Infiltration of optic nerve | |||
Yes | 10 | 18 | |
No | 15 | 8 | |
Total | 25 | 26 | 0.036* |
Differentiation | |||
Good or moderate | 20 | 20 | |
Poor | 5 | 6 | |
Total | 25 | 26 | 0.789 |
Calcification | |||
Yes | 10 | 9 | |
No | 15 | 17 | |
Total | 25 | 26 | 0.691 |
Necrosis | |||
Yes | 14 | 16 | |
No | 11 | 10 | |
Total | 25 | 26 | 0.688 |
Variable | High expression | Low expression | P value |
---|---|---|---|
Age | |||
> 2 years old | 8 | 7 | |
≤ 2 years old | 18 | 18 | |
Total | 26 | 25 | 0.828 |
Gender | |||
Male | 14 | 10 | |
Female | 12 | 15 | |
Total | 26 | 25 | 0.322 |
Laterality | |||
Unilateral | 25 | 24 | |
Bilateral | 1 | 1 | |
Total | 26 | 25 | 0.977 |
Enucleated eye | |||
Right | 9 | 13 | |
Left | 17 | 12 | |
Total | 26 | 25 | 0.210 |
IIRC stage | |||
Group D | 15 | 6 | |
Group E | 11 | 19 | |
Total | 26 | 25 | 0.015* |
High-risk pathology | |||
Yes | 13 | 20 | |
No | 13 | 5 | |
Total | 26 | 25 | 0.025* |
Infiltration of choroid | |||
Yes | 5 | 15 | |
No | 21 | 10 | |
Total | 26 | 25 | 0.003* |
Infiltration of optic nerve | |||
Yes | 9 | 19 | |
No | 17 | 6 | |
Total | 26 | 25 | 0.003* |
Differentiation | |||
Good or moderate | 6 | 5 | |
Poor | 20 | 20 | |
Total | 26 | 25 | 0.789 |
Calcification | |||
Yes | 12 | 7 | |
No | 14 | 18 | |
Total | 26 | 25 | 0.180 |
Necrosis | |||
Yes | 19 | 11 | |
No | 7 | 14 | |
Total | 26 | 25 | 0.035* |