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Japanese Journal of Ophthalmology
Erschienen in: Japanese Journal of Ophthalmology 5/2022

31.05.2022 | Laboratory Investigation

Differential gene expression analysis using RNA sequencing: retinal pigment epithelial cells after exposure to continuous-wave and subthreshold micropulse laser

verfasst von: Tomoyasu Shiraya, Fumiyuki Araki, Suguru Nakagawa, Takashi Ueta, Kiyohito Totsuka, Hitoshi Abe, Yasuyuki Naito, Taku Toyama, Koichiro Sugimoto, Satoshi Kato

Erschienen in: Japanese Journal of Ophthalmology | Ausgabe 5/2022

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Abstract

Purpose

Subthreshold micropulse laser (SMPL) is more clinically efficient for the treatment of diabetic macular edema (DME) than the conventional continuous-wave (CW) laser. We aimed to characterize transcriptome changes after the application of these lasers and to compare the transcripts.

Methods

Human pluripotent stem cell-derived retinal pigment epithelial cells were exposed to laser irradiation. Differentially expressed genes (DEGs), distribution of heat shock protein (Hsp) family, gene expression profile, and gene ontology (GO) enrichment analysis based on RNA sequencing data were investigated at 3 h and 24 h after irradiation.

Results

CW laser induced more DEGs than SMPL (1771 vs. 520 genes). The expression of the Hsp family was confirmed in both groups: however, the induction patterns was different for different genes. GO enrichment analysis revealed that CW laser upregulated the expression of DEGs involved in vasculature development (GO: 0001944), related to apoptosis and repair after cell injury whereas SMPL upregulated the expression of DEGs involved in photoreceptor cell maintenance (GO: 0045494), photoreceptor cell development (GO: 0042461), and sensory perception of light stimuli (GO: 0050953).

Conclusions

The results provide insights into the genetic responses and may contribute to the understanding of the molecular mechanisms of laser-induced thermal effects.
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Metadaten
Titel
Differential gene expression analysis using RNA sequencing: retinal pigment epithelial cells after exposure to continuous-wave and subthreshold micropulse laser
verfasst von
Tomoyasu Shiraya
Fumiyuki Araki
Suguru Nakagawa
Takashi Ueta
Kiyohito Totsuka
Hitoshi Abe
Yasuyuki Naito
Taku Toyama
Koichiro Sugimoto
Satoshi Kato
Publikationsdatum
31.05.2022
Verlag
Springer Japan
Erschienen in
Japanese Journal of Ophthalmology / Ausgabe 5/2022
Print ISSN: 0021-5155
Elektronische ISSN: 1613-2246
DOI
https://doi.org/10.1007/s10384-022-00925-0

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