Introduction
Low bone mass and micro-architectural disturbance of bone tissue are characteristics of osteoporosis, a systemic metabolic illness that increases bone fragility and fracture susceptibility [
1]. Osteoporosis (OP) disproportionately affects elderly and postmenopausal women. Twenty-five percent of patients over 50 who suffer an osteoporotic hip fracture are predicted to pass away within a year [
2]. A total of 200 million people globally are at danger for osteoporosis-related fractures, which can increase morbidity and mortality and have a considerable financial impact on the health care system [
3,
4]. China has entered an aging society in 1999. According to the Chinese Academy of Social Sciences in 2013, China's elderly population has exceeded 200 million, accounting for about 15.5% of the total population, and will grow at a rate of 1 million per year by 2025 [
5]. Xinjiang is located in the northwest border of China, as early as 1995 into the aging society. Curtis [
6] pointed out that the impact of gender on bone mass is mainly related to osteoclasts, osteoblasts. Many experiments have shown that estrogen can promote bone growth at the same time [
7]. Horiuchi et al. [
8] showed that the intake of soybeans was positively correlated with BMD in postmenopausal women. Since there are much more postmenopausal patients than non-postmenopausal patients, the decline in estrogen levels in postmenopausal patients is what causes OP. Rozenberg [
9] recommended using menopausal hormone treatment in women who just went through menopause and had minimal baseline risks for breast cancer, thromboembolic disease, cardiovascular disease, and cerebrovascular illness in order to maintain bone health and prevent future fractures. Furthermore, LRP5 is known to act as a protective factor for type-2 diabetes mellitus (T2DM), promoting insulin signaling in addition to increasing insulin production and lowering blood glucose, and the expression of LRP5 is critical for bone formation, especially osteoblasts [
10]. It was found that the interaction between LRP5 and LRP6 gene polymorphisms, menopausal age, and blood glucose level in postmenopausal populations could increase the risk of OP. At present, there are no reports on the relationship between the polymorphisms of LRP5 gene rs7125942 and rs3736228 in postmenopausal women and systemic metabolic reduction diseases. Our team's previous research also showed that the expression of polymorphisms of LRP5 gene loci in postmenopausal women is related to BMD abnormalities. Therefore, the aim of this study was to evaluate BMD status and major affecting factors in the middle-aged and aging population in Xinjiang and exploring the relationship between LRP5 gene polymorphisms and mutations and BMD is expected to reveal new biological pathways and lay a foundation for the prevention and treatment of OP.
Discussion
OP is a common disease in middle-aged and elderly patients, and the most serious complication is the occurrence of fracture. Shihezi, Xinjiang is a city of army reclamation, it is located in the northwestern border of China and entered the aging society as early as 1995 [
13]. OP has grown to be a significant public health issue. The relationship between gene polymorphisms and mutations in LRP5, the classical receptor of WNT signaling pathway, and glucose, lipid metabolism, and bone mass reduction has also become a research hotspot in recent years.
Our study showed that increasing age is a risk factor for osteopenia and osteoporosis, Clinical Update on Osteoporosis concluded that the key factors affecting OP fractures included a low body mass index (20 kg/m2 or 127 pounds), current smoking, a history of osteoporotic fractures in the family, early menopause (age of < 40 years old) and other factors [
14]. At present, numerous investigations have demonstrated that one of the persistent consequences of DM is OP [
15‐
17]. However, our study showed that BMI and the presence or absence of diabetes were not significantly related to bone mass abnormalities, which may be related to sample selection.
Hutchison et al. [
18] found that 50% of the patients had histological changes in the bone when half of the normal glomerular filtration rate was achieved. Our study confirmed that renal function indicators of serum Cr, serum BUN in the OP group, osteopenia group, and controls group decreased, respectively. A cross-sectional study of older men found that BMD levels in the normal high values of serum UA were higher than that in the normal low values [
19]. Studies have demonstrated that UA can result in intracellular mitochondrial malfunction, which lowers the generation of adenosine triphosphate and negatively affects endothelial function, thereby affecting blood supply to bone tissue and significantly reducing bone turnover [
20]. Our findings demonstrated that UA levels were successively increased in the OP, osteoporosis and control groups, suggesting that controlling UA levels to normal high values could reduce or delay the development of OP. Our study showed that higher serum ALP was associated with lower femoral BMD, which was similar to Filippo Migliorini’s [
21].
The results of our study found that there was statistically significant differences in the distribution of wild-type (CC) and mutant (CG) groups with normal bone mass and abnormal bone mass groups in rs7125942 locus (
P < 0.05). It was suggested that gene mutations in rs7125942 may increase the risk of OP. At rs7125942 locus, compared with wild-type (CC), mutant (CG) had lower FPG and LDL in the normal bone mass group, and lower serum ALP in the bone abnormality group. At rs3736228 locus, the BMD (Femoral neck) of mutant-type (CT/TT) was lower than that of wild-type (CC) in the normal bone mass group (
P < 0.05). The results of multiple linear regression analysis showed that BMI was positively correlated with BMD of femoral neck and lumbar L1-4, and the increase of BMI within a certain range was of positive significance for delaying OP progression, which was consistent with the results of Fan [
22], but this was contrary to the conclusion of Wang et al. [
23], indicating that the increase of BMI beyond a certain range may be used as a risk factor for OP. Decreased TG levels are positive for delaying bone loss, and this conclusion is that, in contrast to Aleidi et al. [
24], increased age and menopausal years are risk factors for OP.
In summary, Xinjiang area is a high prevalence area of OP, and especially in postmenopausal women. Mutations in the LRP5 gene rs7125942 may be related to bone metabolism in postmenopausal women. In this study, the influencing factors of bone metabolism disorders in postmenopausal women in Shihezi area of Xinjiang were explored from the genetic level. The limitation of this study is that we only selected postmenopausal women as the research subjects, and its findings may have limited generalizability beyond the population of postmenopausal women, and bone density may be affected by racial genetic differences, diet, and region. In addition, it is necessary to expand the population sample to explore the effects of gender and sex hormones on bone metabolism, and treatments and interventions for osteopenia need to be further studied. This study provides a basis for gene targeted prevention and treatment of OP.
Conclusion
Meanwhile, the effects of serum ALP, TG, BUN, Cr, and P levels and abnormal renal function on BMD should be emphasized; measurement of L2 BMD is more meaningful in the diagnosis of OP in Xinjiang, China. LRP5 gene rs7125942 site mutation is related to BMD.
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