Background
Marfan syndrome (MFS) is a rare disease often associated with the involvement of the oral and maxillofacial region [
1]. It is an autosomal dominant inherited connective tissue disorder, with an incidence of 1 in 10.000 cases for both sexes [
2]. The syndrome is based on a mutation in the fibrillin-1 gene (FBN1) on chromosome 15q21 [
1]. FBN1 encodes the protein fibrillin-1, an essential component of microfibrils in the extracellular matrix, both in elastic and non-elastic connective tissue [
3,
4]. Accordingly, mutations are associated with tissue weakness, increased signaling of transforming growth factor β, and loss of cell-matrix interactions, ultimately leading to the various phenotypic manifestations of MFS [
5].
In addition to general complications affecting the cardiovascular and musculoskeletal system, eyes, or lungs, MFS is associated with multiple oral manifestations [
2,
6]. These include a narrow and high palate, maxillary and mandibular retrognathia, general malocclusion, crowding of the teeth, irregularities in the number of teeth, and TMD [
6‐
8].
TMD comprises a group of disorders affecting the temporomandibular joint and surrounding structures [
9]. Symptoms may include limited or painful jaw movement, neck pain, and cracking or rubbing of the temporomandibular joint(9). The increased presence of TMD could be the hypermobility of the joints, which is one of the common MFS symptoms. Previous studies in patients with generalized joint hypermobility (GJH) had demonstrated an increased incidence of non-painful subtypes of TMD [
10]. In a GJH cohort (consisting of MFS and Ehlers-Danlos Syndrome (EDS) participants), an association between GJH and TMD was also demonstrated, showing myofascial pain in 69% of participants [
11].
Previous studies have shown that both the presence of TMD and a diagnosis of Marfan syndrome can negatively affect OHRQoL. The TMJ pain, limited jaw mobility and other symptoms caused by TMD can often lead to physical and psychosocial impairment which can significantly affect the quality of life of those affected [
12,
13]. In Marfan syndrome, oral symptoms can also have a negative impact on oral health and quality of life, leading to pain [
6]. It is important to understand the specific effects of these two conditions on the OHRQoL in order to develop appropriate treatment strategies that can not only alleviate symptoms but also improve the quality of life of those affected. No studies have investigated the possible correlation between MFS, TMD, and oral health-related quality of life (OHRQoL). Only information about the prevalence of signs and symptoms of TMD in persons with MFS are available [
14]. Accordingly, this explorative study aims to analyze to what extent persons with MFS are affected by TMD and how this impacts their quality of life.
Materials and methods
Recruitment and study design
An online questionnaire was used to collect data from participants. At the start, the participants were informed about the study contents and had to provide their consent to participate. It was available to participants from Germany, Austria, and Switzerland. Participants were recruited with the help of the Marfan Hilfe (Germany) e.V. (support group; NGO) by publishing a link to the questionnaire on their website. Additionally, the questionnaire was shared in different social media groups where people with the disease come together and exchange their experiences.
Eligibility criteria
The requirements to participate were a minimum age of 18 years and the presence of MFS. The diagnosis had to be confirmed clinically or human genetically (or both). Only questionnaires in which all free-text questions were answered were included. In cases where data were missing from the validated questionnaires, these questionnaires were not regarded during the analysis. In the case of minor typographical mistakes, the questionnaires were included for evaluation if the answer could be inferred with certainty from the context. Those cases were resolved by discussion by two independent authors (T.J. and O.O).
Data collection
The Data were collected over two months (June 2022-July 2022). A questionnaire consisting of free-text questions related to general participant information, oral health, and TMD symptoms was developed. A translated version of the questionnaire can be found in Supplementary File 1. Additionally, three validated questionnaires were used, the German short form of the Oral Health Impact Profile (OHIP-G14) [
15], the German version of the Depression Anxiety Stress Scale (DASS) [
16], and the German version of the Graded Chronic Pain Status, “Graduierung chronischer Schmerzen (GCS) [
17,
18].
Evaluation of oral health and symptoms
Oral health and symptoms were recorded using free-text questions and selectable choices. Participants were asked how many teeth they had already lost and how many times they had been to the dentist in the previous 12 months. Participants could also tick the oral symptoms that applied to them, with multiple answers possible. The possible answers were absence or reduced effect of local anesthesia, pain of the masticatory muscles, parodontitis, hypodontia, disproportion of upper and lower jaw, cleft lip and palate, high palate, shape anomaly of the teeth, malformation of the tooth structure, dislocation of the temporomandibular joint and no oral involvement (see Supplementary File 1).
TMD-like symptoms
As the study was not accompanied by a clinical examination, it was not possible to categorize participants with or without TMD on the basis of Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) [
19]. However, in order to provide a subdivision of participants with and without TMD-like symptoms, two groups were formed. One group consisted of participants who reported that at least one movement of the lower jaw (opening, closing, sideways movement or chewing) was associated with pain or restriction, or in whom at least one masticatory muscle group (cheek, jaw angle, temple) was tense or painful. Participants who had already been diagnosed with TMD were also included in this group. The other group consisted of participants who had none of the above symptoms and had not been diagnosed with TMD.
Evaluation of OHRQoL
To evaluate the OHRQoL, the OHIP-G14 was used [
15]. It consists of 14 questions and provides an overview of the psychosocial impairment of oral health. The questions are answered with a frequency score (“never” = 0, “hardly ever” = 1, “once in a while” = 2, “often” = 3, “very often” = 4). According to current recommendations, the OHIP-G14 was scored according to the four OHRQoL dimensions Oral Function, Orofacial Pain, Orofacial Appearance, and Psychosocial Impact [
20]. For this purpose, only Physical Disability, Physical Pain, Psychological Discomfort, and Handicap domain scores were calculated and interpreted as Oral Function, Orofacial Pain, Orofacial Appearance, and Psychosocial Impact scores, respectively [
20]. As each domain consists of two items, each with a possible score between 0 and 4, the dimensional scores could reach from 0 to 8 points, respectively. Additionally, a summary score of all items was calculated.
Psychological impairment
To evaluate the psychological impairment, the DASS was used [
16]. The questionnaire consists of 21 questions, of which seven items are assigned to the three scales depression, anxiety, and stress. The answer indicates how much a statement applies or does not apply (“Did not apply to me at all - never” = 0, “Applied to me to some extent - sometimes” = 1, “Applied to me to a considerable extent - quite often” = 2, “Applied to me very much - most of the time” = 3). The scores are added separately for each of the three scales. The cut-off value for depression (increased likelihood of a depressive disorder) is 10, for anxiety, a cut-off value of 6, and 10 for stress.
Chronic pain graduation
For grading the level of chronic pain, the GCS was used [
17,
18]. The questionnaire consists of seven questions, four referring to pain-related impairments and three to pain intensity. Responses are scored on a scale ranging from 0 to 10 (0 = no pain, 10 = pain could not be worse, or 0 = no impairment, 10 = you were unable to do anything) and a specification of days on which the everyday activities could not be performed in the last six months due to pain (question 1). For the evaluation, only questions 1, 5, 6, and 7 are to be considered, and the numbers given in the questions are converted into impairment points. An evaluation of questions 2, 3, and 4 (characteristic pain intensity) is only necessary if the sum of the calculated impairment points (from questions 1, 5, 6, and 7) is less than 3. Ultimately, the impairment points are categorized into grades 0–4. Grade 1 is classified as “low impairment - low pain intensity”, grade 2 as “low impairment - high pain intensity”, grade 3 as “severe impairment - moderate limitation” and grade 4 as “severe impairment - high limitation”. Grade 0 can be considered as “no pain”. Grades 1 and 2 are deemed persistent functional pain, and grades 3 and 4 are considered dysfunctional chronic pain.
Statistic methods
The study was conducted to be fully explorative. Therefore, no power analysis was done a priori, and all results were interpreted as hypothesis generating.
To evaluate differences between participants with and without TMD symptoms, categorical variables (age, sex, country) were analyzed using a Chi-square Test. In addition, continuous variables (time of diagnosis, time between first symptoms and diagnosis) were analyzed using a Mann-Whitney-U Test. Differences in DASS and GCS scores between the two groups were analyzed using a Chi-square Test. OHRQoL and the four dimensions of OHRQoL were analyzed using a Mann-Whitney-U test. Differences between the levels of chronic pain on OHRQoL were tested via Kruskal-Wallis Test. The impact of psychological impairment on OHRQoL was analyzed using Mann-Whitney-U Test. All tests were performed at a significance level of α = 5%. Statistical analyses were performed using IBM SPSS Statistics for Mac, Version 28.0.1.0 (IBM Corp., Armonk, NY, USA) and graphics were designed using RStudio Version 2022.07.1 + 554 (RStudio PBC, Boston, MA, USA).
Discussion
This study aimed to analyze to what extent patients with Marfan syndrome are affected by TMD, especially the impact on oral health-related quality of life. A high number of participants in this study showed typical symptoms that occur in TMD. Over half of the participants named clenching or grinding with their teeth or pain in at least one of the masticatory muscles as one of their symptoms. These symptoms may be related to joint hypermobility similar to Ehlers-Danlos syndromes, in which some subtypes also show a high prevalence of TMD symptoms [
10,
11,
21‐
23]. Previous studies have shown similar results for patients with Marfan syndrome experiencing TMD-like symptoms. For example, de Coster et al. studied a cohort of 42 individuals with generalized joint hypermobility (GJH), of which 24 were diagnosed with Marfan syndrome. A positive correlation between GJH and TMD was shown, and 71.4% of all participants showed signs of TMD [
11]. This is in accordance with Bauss et al., who surveyed 281 individuals with Marfan syndrome and concluded that TMD is a critical aspect in affected individuals, with over half of the participants showing symptoms of TMD [
14]. Furthermore, Staufenbiel et al. concluded that the prevalence of temporomandibular joint disorders seems to be higher in Marfan patients than in patients without MFS [
24]. This prevalence of TMD can be seen as reasonably high compared to the general population, which ranges from 15 to 31%, according to multiple studies [
25,
26]. However, only 14.5% of participants in our cohort had already been diagnosed with TMD. This low number seems underdiagnosed since more participants showed TMD-like symptoms (65.8%). This suggests that greater focus should be placed on the diagnosis of TMD in individuals with Marfan syndrome to provide adequate therapy.
One of the main symptoms of TMD is pain in the orofacial region. In our cohort, most participants reported pain ranging from low impairment and intensity to severe impairment and intensity. According to a systematic review of chronic pain in MFS, the prevalence of chronic pain ranged from 47–92% [
27]. Pain is considered chronic if it persists longer than 3 to 6 months or recurs [
28]. Only one study has focused on pain in the temporomandibular region [
14]. Results from this study are comparable to ours, as more than half of the participants reported pain in the TMJ area. The assessment of chronic pain in our cohort showed an increase in pain severity with the presence of TMD symptoms. None of the participants without TMD symptoms showed dysfunctional chronic pain on the chronic pain assessment. Pain in its chronic form can cause various problems, including “chronic fatigue, sleep disturbances, excessive rest, withdrawal from activity, reduced sexual activity, compromised immune function and mood disorders” [
27,
29]. Speed et al. concluded that MFS should be regarded as a chronic pain disorder and can lead to “profound disability and psychological burden” [
30].
The results of our study suggest this, as a considerable number of participants showed an increased susceptibility to depression, anxiety or stress disorders (18.3%, 31.0%, and 21.1%, respectively). Peters et al. also reported significant depressive symptoms in over 40% of an MFS cohort [
31]. The results of other studies showed similar impairments in MFS regarding anxiety, depression, mental fatigue, and other psychiatric symptoms [
30,
32‐
36]. Our data show that TMD symptoms may be associated with psychological impairment. It is essential that the psychological findings are taken seriously but that this does not lead to a psychiatric misdiagnosis. Patients with Marfan syndrome who initially received a psychiatric diagnosis waited an average of 14 years until the correct diagnosis of Marfan syndrome was made [
37]. To date, no comparable studies address the impact of TMD on psychological impairment in individuals with MFS. However, Yap et al. reported that “Participants with […] TMD symptoms generally exhibited significantly higher levels of psychological distress and worse OHRQoL” [
38].
Previous studies have shown the impact of MFS and its psychological impairment on Quality of Life (QoL) [
33‐
36,
39‐
41]. However, only one study investigated the OHRQoL in people with MFS in Germany, and the results suggested that people with MFS show a reduced OHRQoL (OHIP-G14: 13.65 ± 13.53 ) compared to the general population [
6]. When considering the median OHIP value in our cohort, the score was comparably better (median of 6 (IQR 15)). However, this value also showed a reduced OHRQoL compared to the German general population. John et al. observed that 80% of the naturally toothed participants in a representative study had an OHIP-G14 value of ≤ six [
42].
The OHRQoL of people with MFS was reduced in our study, with the median OHIP of people with TMD symptoms being 10.5 points higher than that of people without these symptoms. Similar results were reported by Hanna et al. [
43]. A reduced OHRQoL was observed in their cohort of Australian adults who reported TMD experience. To better assess the extent of the eight-point difference in our cohort, one can refer to results from a study by Reissmann et al. For the OHIP-G14, one point difference was associated with nearly two impacts on OHRQoL per day [
44]. Reissmann et al. found that individuals with TMD had at least three times the impact on OHRQoL as individuals without TMD [
44,
45]. This considerably high impact of TMD on OHRQoL is supported by the results of several studies [
46‐
49]. In a case-control study by Bayat et al., the most important predictors of OHRQoL were an increased level of chronic pain and psychological impairment [
50]. With increasing levels of chronic pain, participants in our cohort also showed a reduction in OHRQoL. Noticeable higher values for the dimension of Oral Function, Orofacial Pain, and Orofacial appearance were observed for the group with TMD symptoms. The presence of symptoms indicating depression, anxiety or stress disorder also led to a reduced OHRQoL.
The EurordisCare surveys have shown that the need for dental care (64%) is very high in people with Marfan syndrome [
37]. Based on this and the findings of our study, several approaches seem to be needed to improve the oral health and related impairments of people affected by MFS. A vital role in this context should be the diagnosis and adequate treatment of TMD to improve OHRQoL. Our data may indicate that the occurrence of TMD in MFS may be overlooked to a considerable extent. Only five of the already diagnosed participants reported being in TMD-specific treatment, of which only two felt an improvement. No studies available demonstrate the potential effects of TMD-specific therapy in MFS. In the general population, a combination of conservative therapies relieved pain in 50–90% of patients [
26]. Therefore, it seems necessary to investigate whether targeted non-invasive therapies can improve the situation. Several studies have recommended using non-invasive therapies such as pharmacotherapy, physiotherapy, and occlusal splints. Self-care, patient education, and cognitive behavior therapy should also not be neglected [
21,
26,
51].
Limitations
One limitation of this study is the small sample size; however, MFS is a rare disease that is not frequently encountered. Within participants, there was a considerable gender imbalance, with over 80% of participants being female. However, in a cohort of participants affected by rare diseases, Bohner et al. showed no impact of gender on OHRQoL [
52]. In addition, the data were collected using an online questionnaire, meaning only the participants’ subjective assessments can be reported. As this is a cross-sectional study, the results should be interpreted with caution and no causal conclusions should be drawn. This would require further case-control studies. Clinical studies with more participants are also needed to verify these subjective findings.
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