Glycemic, insulinemic and incretin responses after oral trehalose ingestion in healthy subjects

Trehalose is a non-reducing disaccharide that consists of two glucose units linked by a α, α-1,1-glucosidic bond. Trehalose is widespread in nature such as plants, fungi and insects, and helps to preserve the life of plants and animals [1, 2]. Although trehalose was previously consumed from natural sources, it has been manufactured from starch through a proprietary enzymatic process since 1995. As a result, trehalose is widely used in a variety of foods to protect against dryness, freezing and osmotic pressure stresses.

In previous studies, we showed that trehalose intake prevented adipocyte hypertrophy and mitigated insulin resistance in mice fed a high-fat diet [3, 4]. In addition, it has been reported that trehalose has various biological effects such as suppression of bone resorption [5] and inflammatory response [6], induction of autophagy [7, 8] and alleviation of Huntington’s disease [9]. Although these effects were found in vitro or in vivo studies, there was little information available about the physiological functions of trehalose in humans.

Oku et al. have reported that trehalose is a carbohydrate that does not raise blood glucose levels quickly and induces lower insulin secretion compared with glucose in female students (age 21.8 ± 5.4) [10]; however, data from a broad age-range of people, including men, remain unknown. Furthermore, the incretin response related to glucose metabolism following trehalose intake has not been examined in human subjects. Incretins are gut hormones that are secreted from enteroendocrine cells into the blood after eating and promote insulin secretion [11]. Gastric inhibitory polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) are main incretins in humans. In recent years, incretin receptor has been detected in many organs, and its various physiological extrapancreatic effects have received a lot of attention [12]. For example, it is known that GIP promotes bone formation [13] and fat accumulation in adipose tissue [14]. This incretin also has been reported to suppress gastric acid secretion in the gastrointestinal tract [15]. In addition, GLP-1 promotes cardioprotection in the heart [16] and delay gastric emptying in the gastrointestinal tract [17]. In this study, we examined glycemic, insulinemic and incretin responses after oral trehalose ingestion in healthy human subjects.

As a result of experiments in healthy human subjects, both blood glucose and insulin levels were found to be significantly lower following trehalose loading compared with glucose loading. Furthermore, active GIP levels were markedly lower following trehalose loading compared with glucose loading. Sex differences were not found in these responses (data not presented).

Diarrhea symptom was observed in the two subjects when 25 g of trehalose were dissolved in 100 mL water and administered in this study. Whereas sixty German subjects were given a 50 g dose of trehalose, on the other hand, diarrhea and malabsorption were not observed in any of the subjects as reported by Bolte et al. [18]. In their study, 400 mL of water was used to administer 50 g of trehalose. In case of Oku et al. [10], furthermore, several dosages of trehalose dissolved in 100–150 mL of tap water were given and no subjects had diarrhea when administered at least 30 g of trehalose. Together with these results, it is possible that diarrhea symptom is caused by the concentration of trehalose solution when given to subject but not total quantity.

Individual blood glucose IAUC after trehalose ingestion was lower than after glucose ingestion. Although a blood glucose IAUC ratio of trehalose loading to glucose loading was individually different, the mean of these values for all subjects was 38% in the present study. This value is equivalent to the glycemic index, which was introduced to classify carbohydrate foods according to the degree of postprandial glycemia. In general, low glycemic index foods improve blood glucose control, the blood lipid profile and fibrinolytic activity in diabetic subjects [19, 20]. Furthermore, postprandial spikes in blood glucose can cause damage to fragile blood vessels in the heart, brain, kidneys, eyes and feet [21‐23]. Trehalose might help prevent these risks because it did not rapidly raise blood glucose levels after ingestion, but requires evealuation for potential usefulness as a sweetener for diabetic patients.

Moreover, the mean individual blood insulin IAUC ratio of trehalose loading to glucose loading was 36%. Hyperinsulinemia characterizes impaired glucose tolerance and obesity, and reflects peripheral insulin resistance [24]. Therefore, food materials that do not cause a rapid rise in blood glucose levels and excessive insulin secretion have been expected to prevent onset and progression of lifestyle-related diseases.

Interestingly, active GIP secretion following trehalose loading was conspicuously lower than that of glucose loading. The mean individual blood GIP IAUC ratio of trehalose loading to glucose loading was only 14%. GIP is secreted from K cells, which exist in the upper small intestine, by the stimulation of nutrients such as carbohydrates and lipids [14]. It is thought that ingested trehalose provides little stimulation to K cells during the process of digestion and absorption in the gastrointestinal tract. The lower active GIP concentration found in this study might be one of the mechanisms behind lower insulin release following trehalose ingestion. Recently, a relationship between GIP and obesity has been clarified, and it has been understood that GIP has a physiological role in the nutrient uptake into adipose tissues, thereby linking over nutrition to obesity [14, 25]. Furthermore, it has been reported that GIP receptor-deficient mice fed a high-fat diet were protected from obesity [25]. Restraining GIP signals may lead to the reduction of disease risk such as obesity. In previous reports, we showed that the intake of trehalose suppressed adipocyte hypertrophy and mitigated insulin resistance in mice fed a high-fat diet [3, 4]. Additionally, we confirmed that the consumption of trehalose with a meal three times daily improved glucose tolerance and mitigated the progression of insulin resistance in humans [26]. Low secretion characteristics of GIP may contribute to the suppression of adipocyte hypertrophy and insulin resistance.

In contrast, it is known that GLP-1 protects pancreatic β-cells and suppresses appetite. In this study, although the active GLP-1 levels following trehalose loading were higher than those following glucose loading from 45 to 180 min after ingestion, the levels were very low probably because of rapid disintegration from the enzyme dipeptidyl peptidase-4 (DPP-4). Therefore, we could not argue that trehalose loading led to higher levels of active GLP-1 secretion than glucose loading in this study. A further study is planned in our laboratory.

Recently, DeBosch et al. [8, 27] proposed a model that trehalose inhibits multiple glucose transporter (GLUT) family members through docking in the inward open conformation of glucose transporters. Therefore, trehalose may prevent excessive glucose absorption in the small intestine by inhibiting glucose transporter function. Furthermore van Can et al. [28] reported the implications for postprandial trehalose ingestion reduced glycemic responses in impaired glucose-tolerant subjects. Therefore a consuming trehalose with daily meals may contribute to the maintenance of good health through the prevention of hyperglycemia. Also trehalose may possibly contribute with useful saccharide in prediabetic and type 2 diabetic patients. These possibilities must be resolved in the future.

In conclusion, we have shown that it is hard for trehalose to give rise to hyperglycemia and hypersecretions of insulin and GIP following ingestion. These results indicate that trehalose may be a useful saccharide for good health because of properties that do not stimulate rapid increases in blood glucose and excessive secretion of insulin and GIP promoting fat accumulation.