nach oben

27.04.2024 | RESEARCH

Desflurane alleviates LPS-induced acute lung injury by modulating let-7b-5p/HOXA9 axis

verfasst von: Xiaoyun Shi, Yundie Li, Shibiao Chen, Huaping Xu, Xiuhong Wang

Einloggen, um Zugang zu erhalten

Abstract

Acute lung injury (ALI) is characterized by acute respiratory failure with tachypnea and widespread alveolar infiltrates, badly affecting patients’ health. Desflurane (Des) is effective against lung injury. However, its mechanism in ALI remains unknown. BEAS-2B cells were incubated with lipopolysaccharide (LPS) to construct an ALI cell model. Cell apoptosis was evaluated using flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was employed to examine the levels of inflammatory cytokines. Interactions among let-7b-5p, homeobox A9 (HOXA9), and suppressor of cytokine signaling 2 (SOCS2) were verified using Dual luciferase activity, chromatin immunoprecipitation (ChIP), and RNA pull-down analysis. All experimental data of this study were derived from three repeated experiments. Des treatment improved LPS-induced cell viability, reduced inflammatory cytokine (tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6)) levels, decreased cell apoptosis, down-regulated the pro-apoptotic proteins (Bcl-2-associated X protein (Bax) and cleaved caspase 3) expression, and up-regulated the anti-apoptotic protein B-cell-lymphoma-2 (Bcl-2) expression in LPS-induced BEAS-2B cells. Des treatment down-regulated let-7b-5p expression in LPS-induced BEAS-2B cells. Moreover, let-7b-5p inhibition improved LPS-induced cell injury. let-7b-5p overexpression weakened the protective effects of Des. Mechanically, let-7b-5p could negatively modulate HOXA9 expression. Furthermore, HOXA9 inhibited the NF-κB signaling by enhancing SOCS2 transcription. HOXA9 overexpression weakened the promotion of let-7b-5p mimics in LPS-induced cell injury. Des alleviated LPS-induced ALI via regulating let-7b-5p/ HOXA9/NF-κB axis.

Graphical Abstract

Nur mit Berechtigung zugänglich

Long ME, Mallampalli RK, Horowitz JC. Pathogenesis of pneumonia and acute lung injury. Clin Sci (Lond). 2022;136(10):747–69. https://doi.org/10.1042/cs20210879.CrossRefPubMed

Mowery NT, Terzian WTH, Nelson AC. Acute lung injury. Curr Probl Surg. 2020;57(5): 100777. https://doi.org/10.1016/j.cpsurg.2020.100777.CrossRefPubMed

Chen X, Wang H, Jia K, Wang H, Ren T. Anti-Semaphorin-7A single chain antibody demonstrates beneficial effects on pulmonary inflammation during acute lung injury. Exp Ther Med. 2018;15(3):2356–64. https://doi.org/10.3892/etm.2018.5724.CrossRefPubMedPubMedCentral

Gorman EA, O’Kane CM, McAuley DF. Acute respiratory distress syndrome in adults: diagnosis, outcomes, long-term sequelae, and management. Lancet. 2022;400(10358):1157–70. https://doi.org/10.1016/S0140-6736(22)01439-8.CrossRefPubMed

Goligher EC, Costa ELV, Yarnell CJ, Brochard LJ, Stewart TE, Tomlinson G, et al. Effect of lowering Vt on mortality in acute respiratory distress syndrome varies with respiratory system elastance. Am J Respir Crit Care Med. 2021;203(11):1378–85. https://doi.org/10.1164/rccm.202009-3536OC.CrossRefPubMed

Woo JH, Baik HJ, Kim CH, Chung RK, Kim DY, Lee GY, et al. Effect of propofol and desflurane on immune cell populations in breast cancer patients: a randomized trial. J Korean Med Sci. 2015;30(10):1503–8. https://doi.org/10.3346/jkms.2015.30.10.1503.CrossRefPubMedPubMedCentral

Lin X, Ju YN, Gao W, Li DM, Guo CC. Desflurane attenuates ventilator-induced lung injury in rats with acute respiratory distress syndrome. Biomed Res Int. 2018;2018:7507314. https://doi.org/10.1155/2018/7507314.CrossRefPubMedPubMedCentral

Wang C, Liu N, Yang HT. Desflurane pretreatment can reduce sepsis-evoked lung injury in rats via inhibiting STAT3 pathway. J Biol Regul Homeost Agents. 2020;34(3):935–42. https://doi.org/10.23812/20-173-a-48.

Tosun M, Olmez H, Unver E, Arslan YK, Cimen FK, Ozcicek A et al. Oxidative and pro-inflammatory lung injury induced by desflurane inhalation in rats and the protective effect of rutin. Adv Clin Exp Med. 2021;30(9):941–8. https://doi.org/10.17219/acem/136194.

10.

Tabnak P, Masrouri S, Geraylow KR, Zarei M, Esmailpoor ZH. Targeting miRNAs with anesthetics in cancer: Current understanding and future perspectives. Biomed Pharmacother. 2021;144: 112309. https://doi.org/10.1016/j.biopha.2021.112309.CrossRefPubMed

11.

Ishikawa M, Iwasaki M, Zhao H, Saito J, Hu C, Sun Q, et al. Sevoflurane and desflurane exposure enhanced cell proliferation and migration in ovarian cancer cells via miR-210 and miR-138 downregulation. Int J Mol Sci. 2021;22(4):1826. https://doi.org/10.3390/ijms22041826.

12.

Ishikawa M, Iwasaki M, Zhao H, Saito J, Hu C, Sun Q, et al. Inhalational anesthetics inhibit neuroglioma cell proliferation and migration via miR-138, -210 and -335. Int J Mol Sci. 2021;22(9):4355. https://doi.org/10.3390/ijms22094355.

13.

Ren J, Wang X, Wei G, Meng Y. Exposure to desflurane anesthesia confers colorectal cancer cells metastatic capacity through deregulation of miR-34a/LOXL3. Eur J Cancer Prev. 2021;30(2):143–53. https://doi.org/10.1097/CEJ.0000000000000608.CrossRefPubMed

14.

Xiao K, He W, Guan W, Hou F, Yan P, Xu J, et al. Mesenchymal stem cells reverse EMT process through blocking the activation of NF-kappaB and Hedgehog pathways in LPS-induced acute lung injury. Cell Death Dis. 2020;11(10):863. https://doi.org/10.1038/s41419-020-03034-3.CrossRefPubMedPubMedCentral

15.

Shen K, Wang X, Wang Y, Jia Y, Zhang Y, Wang K, et al. miR-125b-5p in adipose derived stem cells exosome alleviates pulmonary microvascular endothelial cells ferroptosis via Keap1/Nrf2/GPX4 in sepsis lung injury. Redox Biol. 2023;62: 102655. https://doi.org/10.1016/j.redox.2023.102655.CrossRefPubMedPubMedCentral

16.

Xiao P, Sun S, Cao J, Wang J, Li H, Hou S, et al. Expression profile of microRNAs in bronchoalveolar lavage fluid of rats as predictors for smoke inhalation injury. Burns. 2018;44(8):2042–50. https://doi.org/10.1016/j.burns.2018.07.009.CrossRefPubMed

17.

Wu CY, Ghule SS, Liaw CC, Achudhan D, Fang SY, Liu PI, et al. Ugonin P inhibits lung cancer motility by suppressing DPP-4 expression via promoting the synthesis of miR-130b-5p. Biomed Pharmacother. 2023;167: 115483. https://doi.org/10.1016/j.biopha.2023.115483.CrossRefPubMed

18.

Tang L, Peng L, Tan C, Liu H, Chen P, Wang H. Role of HOXA9 in solid tumors: mechanistic insights and therapeutic potential. Cancer Cell Int. 2022;22(1):349. https://doi.org/10.1186/s12935-022-02767-9.CrossRefPubMedPubMedCentral

19.

Xin X, Yan L, Guangfa Z, Yan H, Keng L, Chunting W. Mesenchymal stem cells promoted lung wound repair through Hox A9 during endotoxemia-induced acute lung injury. Stem Cells Int. 2017;2017:3648020. https://doi.org/10.1155/2017/3648020.CrossRefPubMedPubMedCentral

20.

Li S, Han S, Jin K, Yu T, Chen H, Zhou X, et al. SOCS2 suppresses inflammation and apoptosis during NASH progression through limiting NF-κB activation in macrophages. Int J Biol Sci. 2021;17(15):4165–75. https://doi.org/10.7150/ijbs.63889.CrossRefPubMedPubMedCentral

21.

Shi L, Hu H, Sun P, Li Z, Ji L, Liu S, et al. RPL38 knockdown inhibits the inflammation and apoptosis in chondrocytes through regulating METTL3-mediated SOCS2 m6A modification in osteoarthritis. Inflamm Res. 2022;71(7–8):977–89. https://doi.org/10.1007/s00011-022-01579-x.CrossRefPubMed

22.

Lear TB, McKelvey AC, Evankovich JW, Rajbhandari S, Coon TA, Dunn SR, et al. KIAA0317 regulates pulmonary inflammation through SOCS2 degradation. JCI Insight. 2019;4(19):e129110. https://doi.org/10.1172/jci.insight.129110.

23.

Zhou X, Lu R. HOXA9/MEIS1 targets in leukemia: reinforced signaling networks and therapeutic opportunities. Haematologica. 2023;108(5):1205–7. https://doi.org/10.3324/haematol.2022.281779.CrossRefPubMed

24.

Adi O, Apoo FN, Fong CP, Ahmad AH, Roslan NL, Khan FA et al. Inhaled volatile anesthetic gas for severe bronchospasm in the emergency department. Am J Emerg Med. 2023;68:213.e5-.e9. https://doi.org/10.1016/j.ajem.2023.04.032.

25.

Li P, Gu L, Bian Q, Jiao D, Xu Z, Wang L. Long non-coding RNA MALAT1 enhances the protective effect of dexmedetomidine on acute lung injury by sponging miR-135a-5p to downregulate the ratio of X-box binding proteins XBP-1S/XBP-1U. Bioengineered. 2021;12(1):6377–89. https://doi.org/10.1080/21655979.2021.1967579.CrossRefPubMedPubMedCentral

26.

Yu Y, Zhang Y. Desflurane accelerates neuronal cytotoxicity of Aβ by downregulating miR-214. Neurosci Lett. 2013;554:28–33. https://doi.org/10.1016/j.neulet.2013.08.063.CrossRefPubMed

27.

Chen S, Ding R, Hu Z, Yin X, Xiao F, Zhang W, et al. MicroRNA-34a inhibition alleviates lung injury in cecal ligation and puncture induced septic mice. Front Immunol. 2020;11:1829. https://doi.org/10.3389/fimmu.2020.01829.CrossRefPubMedPubMedCentral

28.

He K, Han S, An L, Zhang J. Inhibition of microRNA-214 alleviates lung injury and inflammation via increasing FGFR1 expression in ventilator-induced lung injury. Lung. 2021;199(1):63–72. https://doi.org/10.1007/s00408-020-00415-5.CrossRefPubMed

29.

Li Z, Yu Y, Liu C, Chen G, Gong W, Luo J, et al. Identification of the key ferroptosis-related genes involved in sepsis progression and experimental validation in vivo. Front Pharmacol. 2022;13: 940261. https://doi.org/10.3389/fphar.2022.940261.CrossRefPubMedPubMedCentral

30.

Liu F, Yang Y, Peng W, Zhao N, Chen J, Xu Z, et al. Mitophagy-promoting miR-138-5p promoter demethylation inhibits pyroptosis in sepsis-associated acute lung injury. Inflamm Res. 2023;72(2):329–46. https://doi.org/10.1007/s00011-022-01675-y.CrossRefPubMed

31.

Zang BB, Li H, Yang Y, Xie H, Xu XT. [The role of miR-135b-5p in inhibiting mice acute lung injury (ALI) induced by sepsis and its mechanism]. Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2022;38(4):366–72. https://doi.org/10.12047/j.cjap.6263.2022.069.

32.

Lambert M, Alioui M, Jambon S, Depauw S, Van Seuningen I, David-Cordonnier MH. Direct and indirect targeting of HOXA9 transcription factor in acute myeloid leukemia. Cancers (Basel). 2019;11(6):837. https://doi.org/10.3390/cancers11060837.

33.

Zhao R, Wang L, Wang T, Xian P, Wang H, Long Q. Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury. J Control Release. 2022;345:214–30. https://doi.org/10.1016/j.jconrel.2022.03.025.CrossRefPubMed

34.

Zhuang C, Kang M, Lee M. Delivery systems of therapeutic nucleic acids for the treatment of acute lung injury/acute respiratory distress syndrome. J Control Release. 2023;360:1–14. https://doi.org/10.1016/j.jconrel.2023.06.018.CrossRefPubMed

35.

Fuller BM, Mohr NM, Skrupky L, Fowler S, Kollef MH, Carpenter CR. The use of inhaled prostaglandins in patients with ARDS: a systematic review and meta-analysis. Chest. 2015;147(6):1510–22. https://doi.org/10.1378/chest.14-3161.CrossRefPubMedPubMedCentral

36.

Redaelli S, Pozzi M, Giani M, Magliocca A, Fumagalli R, Foti G, et al. Inhaled nitric oxide in acute respiratory distress syndrome subsets: rationale and clinical applications. J Aerosol Med Pulm Drug Deliv. 2023;36(3):112–26. https://doi.org/10.1089/jamp.2022.0058.CrossRefPubMed

37.

An L, Zhong Y, Tan J, Liu Y, Li A, Yang T, et al. Sevoflurane exerts protection against myocardial ischemia-reperfusion injury and pyroptosis through the circular RNA PAN3/microRNA-29b-3p/stromal cell-derived factor 4 axis. Int Immunopharmacol. 2023;120: 110219. https://doi.org/10.1016/j.intimp.2023.110219.CrossRefPubMed

38.

Koutsogiannaki S, Okuno T, Kobayashi Y, Ogawa N, Yuki K. Isoflurane attenuates sepsis-associated lung injury. Biochem Biophys Res Commun. 2022;599:127–33. https://doi.org/10.1016/j.bbrc.2022.02.028.CrossRefPubMedPubMedCentral

39.

Fu Z, Wu X, Zheng F, Zhang Y. Sevoflurane anesthesia ameliorates LPS-induced acute lung injury (ALI) by modulating a novel LncRNA LINC00839/miR-223/NLRP3 axis. BMC Pulm Med. 2022;22(1):159. https://doi.org/10.1186/s12890-022-01957-5.CrossRefPubMedPubMedCentral

40.

Schutte D, Zwitserloot AM, Houmes R, de Hoog M, Draaisma JM, Lemson J. Sevoflurane therapy for life-threatening asthma in children. Br J Anaesth. 2013;111(6):967–70. https://doi.org/10.1093/bja/aet257.CrossRefPubMed

41.

Karacaer F, Biricik E, Ilginel M, Lafli Tunay D, Baydar O, Avci A, et al. Effects of ketamine infusion on oxygenation in patients with chronic obstructive pulmonary disease undergoing lung cancer surgery. Turk J Anaesthesiol Reanim. 2023;51(1):16–23. https://doi.org/10.5152/TJAR.CrossRefPubMedPubMedCentral

42.

Antoniu SA. Investigational inhaled therapies for non-CF bronchiectasis. Expert Opin Investig Drugs. 2018;27(2):139–46. https://doi.org/10.1080/13543784.2018.1427728.CrossRefPubMed

43.

Luo X, Miao CH, Ge BX, Jiang H. Effects of isoflurane, sevoflurane and desflurane on expression of ICAM-1 and VCAM-1 in LPS-induced rat lung microvascular endothelial cells. Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010;39(5):464–9. https://doi.org/10.3785/j.issn.1008-9292.2010.05.004.CrossRefPubMed

44.

Cui Y, Qi Y, Ding L, Ding S, Han Z, Wang Y, et al. miRNA dosage control in development and human disease. Trends Cell Biol. 2023;34(1):31–47. https://doi.org/10.1016/j.tcb.2023.05.009.CrossRefPubMed

45.

Li C, Liu JH, Su J, Lin WJ, Zhao JQ, Zhang ZH, et al. LncRNA XIST knockdown alleviates LPS-induced acute lung injury by inactivation of XIST/miR-132-3p/MAPK14 pathway: XIST promotes ALI via miR-132-3p/MAPK14 axis. Mol Cell Biochem. 2021;476(12):4217–29. https://doi.org/10.1007/s11010-021-04234-x.CrossRefPubMedPubMedCentral

46.

Wu W, Zhong W, Xu Q, Yan J. Silencing of long non-coding RNA ZFAS1 alleviates LPS-induced acute lung injury by mediating the miR-96-5p/OXSR1 axis in sepsis. Am J Med Sci. 2022;364(1):66–75. https://doi.org/10.1016/j.amjms.2022.03.008.CrossRefPubMed

47.

Zhang XL, An J, Deng YZ, Fang XZ, Xu CY, Liu XF, et al. A novel miRNA-762/NFIX pathway modulates LPS-induced acute lung injury. Int Immunopharmacol. 2021;100: 108066. https://doi.org/10.1016/j.intimp.2021.108066.CrossRefPubMed

48.

Wang Y, Chen J, Chen W, Liu L, Dong M, Ji J, et al. LINC00987 ameliorates COPD by regulating LPS-induced cell apoptosis, oxidative stress, inflammation and autophagy through let-7b-5p/SIRT1 axis. Int J Chron Obstruct Pulmon Dis. 2020;15:3213–25. https://doi.org/10.2147/copd.S276429.CrossRefPubMedPubMedCentral

49.

Li P, Hao X, Liu J, Zhang Q, Liang Z, Li X, et al. miR-29a-3p regulates autophagy by targeting Akt3-mediated mTOR in SiO₂-induced lung fibrosis. Int J Mol Sci. 2023;24(14):11440. https://doi.org/10.3390/ijms241411440.

50.

Yu H, Lin L, Zhang Z, Zhang H, Hu H. Targeting NF-κB pathway for the therapy of diseases: mechanism and clinical study. Signal Transduct Target Ther. 2020;5(1):209. https://doi.org/10.1038/s41392-020-00312-6.CrossRefPubMedPubMedCentral

51.

Kuhar E, Chander N, Stewart DJ, Jahandideh F, Zhang H, Kristof AS, et al. A preclinical systematic review and meta-analysis assessing the effect of biological sex in lipopolysaccharide-induced acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2024. https://doi.org/10.1152/ajplung.00336.2023.CrossRefPubMed

52.

Yang C, Song C, Liu Y, Qu J, Li H, Xiao W, et al. Re-Du-Ning injection ameliorates LPS-induced lung injury through inhibiting neutrophil extracellular traps formation. Phytomedicine. 2021;90: 153635. https://doi.org/10.1016/j.phymed.2021.153635.CrossRefPubMedPubMedCentral

53.

Hsieh PC, Wu YK, Yang MC, Su WL, Kuo CY, Lan CC. Deciphering the role of damage-associated molecular patterns and inflammatory responses in acute lung injury. Life Sci. 2022;305: 120782. https://doi.org/10.1016/j.lfs.2022.120782.CrossRefPubMed

Titel: Desflurane alleviates LPS-induced acute lung injury by modulating let-7b-5p/HOXA9 axis
verfasst von: Xiaoyun Shi
Yundie Li
Shibiao Chen
Huaping Xu
Xiuhong Wang
Publikationsdatum: 27.04.2024
Verlag: Springer US
Erschienen in: Immunologic Research
Print ISSN: 0257-277X
Elektronische ISSN: 1559-0755
DOI: https://doi.org/10.1007/s12026-024-09474-9

Neu im Fachgebiet HNO

Erhebliches Risiko für Kehlkopfkrebs bei mäßiger Dysplasie

29.05.2024 Larynxkarzinom Nachrichten

Fast ein Viertel der Personen mit mäßig dysplastischen Stimmlippenläsionen entwickelt einen Kehlkopftumor. Solche Personen benötigen daher eine besonders enge ärztliche Überwachung.

Hörschwäche erhöht Demenzrisiko unabhängig von Beta-Amyloid

29.05.2024 Hörstörungen Nachrichten

Hört jemand im Alter schlecht, nimmt das Hirn- und Hippocampusvolumen besonders schnell ab, was auch mit einem beschleunigten kognitiven Abbau einhergeht. Und diese Prozesse scheinen sich unabhängig von der Amyloidablagerung zu ereignen.

„Übersichtlicher Wegweiser“: Lauterbachs umstrittener Klinik-Atlas ist online

17.05.2024 Klinik aktuell Nachrichten

Sie sei „ethisch geboten“, meint Gesundheitsminister Karl Lauterbach: mehr Transparenz über die Qualität von Klinikbehandlungen. Um sie abzubilden, lässt er gegen den Widerstand vieler Länder einen virtuellen Klinik-Atlas freischalten.

Betalaktam-Allergie: praxisnahes Vorgehen beim Delabeling

16.05.2024 Pädiatrische Allergologie Nachrichten

Die große Mehrheit der vermeintlichen Penicillinallergien sind keine. Da das „Etikett“ Betalaktam-Allergie oft schon in der Kindheit erworben wird, kann ein frühzeitiges Delabeling lebenslange Vorteile bringen. Ein Team von Pädiaterinnen und Pädiatern aus Kanada stellt vor, wie sie dabei vorgehen.

Update HNO

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Desflurane alleviates LPS-induced acute lung injury by modulating let-7b-5p/HOXA9 axis

Abstract

Graphical Abstract

Neu im Fachgebiet HNO

Erhebliches Risiko für Kehlkopfkrebs bei mäßiger Dysplasie

Hörschwäche erhöht Demenzrisiko unabhängig von Beta-Amyloid

„Übersichtlicher Wegweiser“: Lauterbachs umstrittener Klinik-Atlas ist online

Betalaktam-Allergie: praxisnahes Vorgehen beim Delabeling

Update HNO

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Graphical Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Neu im Fachgebiet HNO

Erhebliches Risiko für Kehlkopfkrebs bei mäßiger Dysplasie

Hörschwäche erhöht Demenzrisiko unabhängig von Beta-Amyloid

„Übersichtlicher Wegweiser“: Lauterbachs umstrittener Klinik-Atlas ist online

Betalaktam-Allergie: praxisnahes Vorgehen beim Delabeling

Update HNO