Background
Materials and methods
High-throughput expression profiles for GDM
Screening of differentially expressed RNAs
Collection of m6A regulators and identification of m6A-Related DELs
Construction of a lncRNA–miRNA–mRNA network associated with GDM
Identification of the m6A-related subnetwork and module
Construction of a Support-Vector Machine (SVM) classifier
Clinical samples and cell culture
Quantitative real-time polymerase chain reaction (qPCR)
RNA immunoprecipitation (RIP) assay
Statistical analysis
Results
Filtering results for DELs, DEMis, and DEMs
Reference | DEMi name | Location | Trend |
---|---|---|---|
Gillet 2019 [26] | miR-122-5p | Placental exosomes | + |
miR-132-3p | Placental exosomes | + | |
miR-1323 | Placental exosomes | + | |
miR-136-5p | Placental exosomes | + | |
miR-182-3p | Placental exosomes | + | |
miR-210-3p | Placental exosomes | + | |
miR-29a-3p | Placental exosomes | + | |
miR-29b-3p | Placental exosomes | + | |
miR-342-3p | Placental exosomes | + | |
miR-520 h | Placental exosomes | + | |
Ding 2018 [21] | miR-138-5p | Placenta | – |
miR-202-5p | Placenta | + | |
miR-210-5p | Placenta | – | |
miR-3158-5p | Placenta | – | |
miR-4732-3p | Placenta | – | |
Nair 2018 [22] | miR-125a-3p | Placenta, placental-derived exosomes, circulating exosomes and skeletal muscle | + |
miR-99b-5p | Placenta, placental-derived exosomes, circulating exosomes and skeletal muscle | + | |
miR-197-3p | Placenta, placental-derived exosomes, circulating exosomes and skeletal muscle | + | |
miR-22-3p | Placenta, placental-derived exosomes, circulating exosomes and skeletal muscle | + | |
miR-224-5p | Placenta, placental-derived exosomes, circulating exosomes and skeletal muscle | + | |
miR-584-5p | Placenta, placental-derived exosomes | + | |
miR-186-5p | Placenta, placental-derived exosomes | + | |
miR-433-3p | Placenta, placental-derived exosomes | + | |
miR-423-3p | Placenta, placental-derived exosomes | + | |
miR-208a-3p | Placenta, placental-derived exosomes | – | |
miR-335-5p | Placenta, placental-derived exosomes | – | |
miR-451a | Placenta, placental-derived exosomes | – | |
miR-145-3p | Placenta, placental-derived exosomes | – | |
miR-369-3p | Placenta, placental-derived exosomes | – | |
miR-483-3p | Placenta, placental-derived exosomes | – | |
miR-203a-3b | Placenta, placental-derived exosomes | – | |
miR-574-3p | Placenta, placental-derived exosomes | – | |
miR-144-3p | Placenta, placental-derived exosomes | – | |
miR-6795-5p | Placenta, placental-derived exosomes | – | |
miR-550a-3-3p | Placenta, placental-derived exosomes | – | |
miR-411-5p | Placenta, placental-derived exosomes | – | |
miR-140-3p | Placenta, placental-derived exosomes | – | |
Li 2018 [27] | MIR96 | Placenta | – |
Xu 2017 [23] | miR-503 | Placenta | + |
Muralimanoharan 2016 [24] | miR-143 | Placenta | – |
Li 2015 [25] | miR-508-3p | Placenta | + |
miR-27a | Placenta | – | |
miR-9 | Placenta | – | |
miR-92a | Placenta | – | |
miR-30d | Placenta | – | |
miR-362 -5p | Placenta | – | |
miR-502-5p | Placenta | – | |
miR-33a | Placenta | – |
Upregulated | Downregulated | Total | |
---|---|---|---|
GSE19649 | 108 | 35 | 143 |
GSE2956 | 133 | 185 | 318 |
GSEShengjing | 42 | 37 | 79 |
Construction of the ceRNA network
DEL number | Name | Node degree | Trend |
---|---|---|---|
1 | LINC00667 | 5 | Upregulated |
2 | AC016831.1 | 4 | Downregulated |
3 | CARMN | 3 | Downregulated |
4 | AC090114.2 | 3 | Upregulated |
5 | AL589745.1 | 3 | Upregulated |
6 | LINC00639 | 2 | Downregulated |
7 | AP000350.6 | 2 | Upregulated |
8 | AL136962.1 | 1 | Downregulated |
9 | AC106028.3 | 1 | Downregulated |
10 | LINC01483 | 1 | Downregulated |
11 | LINC00921 | 1 | Downregulated |
12 | GMDS-DT | 1 | Upregulated |
13 | GASAL1 | 1 | Upregulated |
14 | LINC00174 | 1 | Upregulated |
15 | AP002360.3 | 1 | Upregulated |
16 | LINC01087 | 1 | Upregulated |
Hub gene | Key module | ||
---|---|---|---|
Annotation | Degree | Annotation | Degree |
MYC | 36 | MYC | 36 |
VEGFA | 32 | VEGFA | 32 |
FN1 | 30 | EP300 | 30 |
EP300 | 30 | FN1 | 30 |
ACTB | 28 | ACTB | 28 |
FOS | 22 | FOS | 22 |
CREB1 | 16 | PTGS2 | 16 |
PTGS2 | 16 | CREB1 | 16 |
THBS1 | 15 | CDKN1A | 11 |
SCD | 15 |