Assessment of knowledge, attitudes and practice towards Vitamin D among university students in Pakistan

There is an increasing body of evidence that suggests vitamin D deficiency is becoming a global epidemic, with South Asians being one of the most affected population groups [1‐3]. Exposure to sunlight is the main source of vitamin D. Some of the best dietary sources of vitamin D include oily fish and egg yolks, as well as vitamin D dietary supplements [4, 5]. Despite receiving plentiful sunlight throughout the year in their countries, South Asians remain one of the most vulnerable populations to suffer from low vitamin D levels [6]. A number of studies that have measured vitamin D levels among South Asian population groups have indicated a high prevalence of its deficiency among healthy young adults, women and infants [7‐9].

Although the biological factors that reduce serum vitamin D levels are known, the knowledge and attitudes towards vitamin D need further investigation especially in dark-skinned population of South Asian countries [10‐12]. Ethno-cultural factors, environmental factors, and behavioural practices which limit sun exposure may contribute to vitamin D deficiency in these populations [13‐15]. These factors are not well-understood and have not been extensively examined especially in a South Asian context.

Pakistan is a sundrenched South Asian country with cutaneous production of vitamin D possible throughout the year. However, the recent national nutrition survey of Pakistan reported a high prevalence of vitamin D deficiency [16‐18]. Studies exploring vitamin D deficiency in Pakistan have identified it to be widespread among various segments of the population including asymptomatic adult population, ambulatory patients, mothers and their newborns [16, 19, 20]. Ninety percent of university students, especially females, aged between 18 and 35 years have been reported as vitamin D deficient [21‐23].

Researchers are calling for public health strategies including supplementation to address high levels of vitamin D deficiency [18, 24]. A recent international report into vitamin D deficiency in the developing world recommended improvement in awareness about vitamin D and its impact of health outcomes among adult population. Nevertheless, there is an absence of studies that examine existing knowledge, attitudes and practices about vitamin D among young adults in high risk countries like Pakistan [3]. Understanding the level of knowledge, attitudes and practice about vitamin D is vital to facilitate policy makers in designing interventions appropriate for the context of Pakistan. The aim of this study therefore was to assess vitamin D related knowledge, attitudes and practices (KAP) among university students in Pakistan. In addition, we also examined possible association of KAP with gender and testing status for vitamin D.

A quantitative cross-sectional design was used to conduct this research. An anonymous survey was designed to examine knowledge, attitudes and practices towards vitamin D among university students in Pakistan. Eligibility criteria included both male and female adults (> 16 years) living in the Pakistan, enrolled in an undergraduate or postgraduate program in a tertiary education institute.

The instrument was developed based on review of previous studies consisting of validated items across range of dimensions, including the assessment of skin colour type, sun exposure, dietary knowledge about vitamin D etc. [25‐27]. The questionnaire consisted of:

Students from universities in Islamabad, Pakistan were invited to participate in the study. Of the 900 eligible students invited to participate, 559 (62%) logged onto the online questionnaire platform. Of these, 505 (56% of 900) completed the questionnaire with full information on sociodemographic characteristics; and, therefore, were included in the final analysis. Fifty-eight percent of participants identified as male and 42% as female. The sample was almost homogeneous in terms of age and education with majority being 16–25 years old (88%) and undergraduate (85%) students. General characteristics of the surveyed population are summarised in Table 1.

Our study is one of the very few which aim at understanding at knowledge, attitudes and practices towards vitamin D among healthy university students in Pakistan, a country marked by very high deficiency of vitamin D across the entire population [24].

Despite this cohort being university students and having access to the internet and health information, knowledge about vitamin D was quite poor. Poor knowledge could be due to conflicting information available about vitamin D and lack of clear localised messages about sun exposure. Similar limitations to the knowledge of vitamin D have been reported in studies across Bangladesh, the Middle East, Iran, Lebanon and also among immigrants from South Asian countries living across Europe. Similar to our study, most studies have found their participants not able to identify sources of vitamin D in food and sunlight as critical enabler in vitamin D production [28, 29]. However, our results are in contrast to those reported in the UK where the participants did demonstrate good level of knowledge about vitamin D [30]. This perhaps is indicative of the increased public awareness and food fortification practices in the developed world. Multi-prong strategies are required to appraise public knowledge and awareness about vitamin D. People should receive information, in local languages, that reflects the actual state of knowledge regarding vitamin D and its association with health, along with clear information on vitamin D sources [31]. These national public health messages should be relayed across various platforms (i.e., social media, health centres, universities, schools), especially through the media, to increase awareness among all sub-groups of the population [31]. Further it is important to recognise awareness regarding the causes and prevention of vitamin D deficiency as an avenue to be explored by the public health offices, which should begin by conducting conclusive studies to determine general public knowledge regarding this pertinent issue [24]. Food Fortification Programme (FFP) in Pakistan is exploring the food fortification of staple foods like milk, vegetable oil and wheat with vitamin D [32, 33]. This is in alignment with evidence that suggests universal vitamin D food fortification can improve serum 25-hydroxyvitamin D levels [34, 35]. However, to encourage public adaptation, it is important to address any public concerns about food fortification, which have been reported to exist especially among South Asians [12, 36]. In addition, an effective strategy to increase awareness may be to conduct awareness campaigns which offer public funded vitamin D level testing for high risk communities.

To our knowledge this is the first study that examined testing and supplementation practices in relevance to vitamin D among young adults in Pakistan. Despite widespread concern about vitamin D levels being low, majority of participants (72%) in our study had never been tested for vitamin D levels. Vitamin D tests are costly in Pakistan and act as a deterrent for people getting tested to check their vitamin D levels [37]. This may indicate why educated young adults would not get tested for vitamin D levels despite their concerns. Getting tested, however, was related to use of supplementation. This is in line with Agens et al. who reported that physicians who had patients vitamin D tested were linked with recommending higher dose of supplement to patients [38]. Therefore, reducing the testing costs to enable timely testing would help in early identification of the vitamin D deficiency. To reduce testing costs, there is an increasing call for developing diagnostic kits locally in Pakistan [39].

Fifty-two percent of participants in our study were not taking any supplements, but among those who reported taking them, females were the more likely users of supplements. This finding may be indicative of prevalent discussions in mainstream media channels that particularly target young women to have adequate vitamin D in early 20s and encourage use of supplements [24, 40]. We recommend extension of these discussions to the general population including the other population groups of the society. It is important to note that low doses were reported by those taking supplements irrespective of the gender with more than half not knowing the dose they were taking. To address the severe deficiency cases, clinicians are recommending higher dose supplementation especially in context of Pakistani population [41]. Although there is a need to timely identify and treat severe vitamin D deficiency, such medicalised view towards vitamin D may highlight the existing culture of testing and treating with tablets among South Asians [12]. This approach may only help in short–term and can be ineffective for addressing this concerning problem for the long term [15, 29, 31, 42, 43].

In terms of sun exposure practices, both male and female participants reported spending similar amount of time outdoors during weekdays. However, time spent outdoors on weekend days differed significantly between males and females. This finding corroborates with studies that have exclusively examined the vitamin D status and reasons for its deficiency among women in Pakistan, and cited lifestyle, the clothing, season, and outdoor activities as primary contributing factors. In our study, 80% of females reported wearing clothing that covers their heads when outdoors, reflective of the Muslim majority population of the country. However, similar to other studies our results did not show vitamin D knowledge, attitudes or practices to be associated with veiling [44, 45]. Therefore, irrespective of the veiling practices, female participants spent less time outdoors on weekends. This perhaps is reflective of the conservative culture and lack of safe outdoor spaces exclusively for women. To address the magnitude of the problem, vitamin D deficiency should be treated like a public health epidemic at national level and introduce public awareness programs like that for iodine deficiency which have been successful in the Pakistani context [46].

The study has several limitations first of which is it was done on a convenience sample and selection bias might be present since the study was carried out in a specific university setting. Hence the results cannot be generalized for the Pakistani population. The limited age group range also adds to the limitations of this study. Future research should validate this questionnaire so that it can be used in a nationwide larger study. Importantly, serum 25-hydroxyvitamin D should be collected so that association between knowledge, attitudes and practices with vitamin D levels can be tested. In addition, further studies should explore ways in which innovative information channels like social media can improve public awareness and their impact using diffusion of innovation framework [47].

The majority of the study participants lacked in knowledge about vitamin D and had inconsistent practices towards managing its deficiency. Despite widespread concern about vitamin D levels being low, testing for vitamin D was not common in our population. Further research, on a larger scale, is needed in this area to enable a better understanding of the knowledge and attitudes about vitamin D in high risk South Asian populations and how interventions like fortified foods or sun exposure advices should be implemented for their long-term effectiveness.