Background
Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder originated by a mixture of etiopathogenic mechanisms, which likely arise from complex interactions among genetics, lifestyle and environmental risk factors. Substantial evidence suggests that the aggregation of amyloid-beta (Aβ) isoforms into senile plaques is the earliest recognizable pathological event in AD. These first Aβ deposits precede overt clinical symptoms by decades [
1,
2] and they supposedly initiate the pathogenic cascade that ultimately leads to cognitive decline [
3]. Identifying risk factors for Aβ deposition in asymptomatic older adults is of paramount importance to understand AD etiology and to ameliorate AD-related brain lesions before cognitive decline appears.
Persistent viral infections have been suggested to play a role in AD pathogenesis, particularly the herpes simplex virus type 1 (HSV-1) [
4‐
7], a ubiquitous neurotropic human pathogen infecting over 67% of the world’s population [
8]. The HSV-1 virus first infects oral and nasal surface epithelia and then travels through retrograde axonal transport to the trigeminal ganglia to establish a latent infection, pointing to ganglionic sensory neurons as a lifelong reservoir of recurrent infections [
9]. A variety of factors, such as stress, inflammation, immunosuppression, fever, hormone imbalance, exposure to ultraviolet light, or injury to the tissues innervated by latently-infected neurons, are able to engage the latent virus in productive replication, affecting HSV-1-infected neurons or surrounding, non-neuronal cells [
10].
Convergent research has led to the hypothesis that cumulative effects of recurrent HSV brain infections may increase AD pathology [
11‐
13]. However, there is lack of human in vivo evidence linking HSV to hallmarks of AD pathology in normal aging, which may contribute to shed light on the role of HSV infection as a potential AD risk factor before the onset of cognitive deficits. To specifically address this question, we have investigated whether increased serum levels of anti-HSV IgG in cognitively normal older adults is associated with increased cortical Aβ load, as revealed by amyloid PET imaging, and/or with blood markers of neurodegeneration, as shown by neurofilament light chain (NfL) and tau phosphorylated at threonine-181 (pTau-181) levels. We expected a positive association between anti-HSV IgG levels and cortical Aβ load based on evidence that HSV infection leads to altered processing of the amyloid precursor protein (APP) [
14] and increases enzymes responsible for Aβ formation [
15].
Postmortem studies have revealed that the majority of Aβ plaques located in fronto-temporal cortices of AD patients and non-demented older individuals contained HSV-1 DNA [
16]. Consequently, associations between anti-HSV IgG and binding of the amyloid PET tracer to Aβ deposits are expected to be mostly restricted to frontal and temporal cortical regions, which may also exhibit altered patterns of functional connectivity in individuals showing the highest levels of anti-HSV IgG. We did not expect associations between anti-HSV IgG levels and blood markers of neurodegeneration, since cognitive deficits are mostly induced by tau pathology occurring subsequently to Aβ deposition in confirmed AD patients [
17,
18].
Gene products of the HSV-1 life cycle have been shown to interact with the apolipoprotein E allele 4 (APOE4), the strongest genetic risk factor in the sporadic form of AD, to instigate both viral infectivity and increase AD risk [
19‐
21]. Based on these findings, we hypothesized that associations between serum levels of anti-HSV IgG and cortical Aβ load would be more evident in older individuals carrying the APOE4 genotype.
Methods
Participants
Sixty-five cognitively normal older adults participated in the study (66.8 ± 5.5 years; age range: 54–76 years; 44 females). They were recruited from senior citizen’s associations, health-screening programs, and hospital outpatient services. All of them underwent neurological and neuropsychological assessment to discard the presence of cognitive impairment and/or dementia. Individuals with medical conditions affecting brain structure or function (e.g., cerebrovascular disease, epilepsy, head trauma, history of neurodevelopmental disorders, mental retardation, alcohol abuse, hydrocephalus, and/or intracranial mass) were not included in the study. Participants met the following criteria: (i) normal global cognitive status in the Mini-Mental State Examination (scores ≥ 26); (ii) normal cognitive performance in the neuropsychological tests relative to appropriate reference values for age and education level; (iii) global score of 0 (no dementia) in the Clinical Dementia Rating; (iv) functional independence as assessed by the Spanish version of the Interview for Deterioration in Daily Living Activities [
22]; (v) scores ≤ 5 (no depression) in the short form of the Geriatric Depression Scale [
23]; and (vi) not be taking medications that affected cognition, sleep, renal and/or hepatic function. All participants gave informed consent to the experimental protocol approved by the Ethical Committee for Clinical Research of the Junta de Andalucía according to the principles outlined in the Declaration of Helsinki.
Neuropsychological assessment
Participants were administered with a comprehensive battery of neuropsychological tests to assess memory, language, attention, processing speed, and executive function skills such as working memory, inhibition, flexibility and planning. Cognitive tests included the Spanish version of the memory binding test (MBT) [
24], the face-name associative memory exam (S-FNAME) [
25], the D2 test, the letter-number sequencing and the digit span subtests of the Wechsler Adult Intelligence Scale-III, the spatial span subtest of the Wechsler Memory Scale-III, the short form of the Boston naming test (BNT), the semantic and phonological fluency tests based on the “Animal” and letter “P” naming tasks, the symbol digit modalities test, the two forms of the trail making test (TMT-A and TMT-B), and the tower of London (TL). All scores were z transformed. Inverse z-values were used when higher scores corresponded to worse performance. We further computed the Spearman’s ‘g’ factor as an index of global cognitive function. This analysis was done with R Statistical Software v3.0.1 (R Foundation for Statistical Computing, Vienna, Austria) using the
prcomp function.
Serological determination of HSV infection status
Fasting blood samples were collected at 9:00–10:00 AM in all participants, immediately processed, and stored at -80ºC until analysis. Serological evaluation of HSV infections were assessed with Enzygnost® enzyme-linked immunosorbent assays (Siemens Healthcare, Marburg, Germany), following the manufacturer’s instructions. These assays allow qualitative detection and quantitative determination of human IgG (respectively IgM) antibodies to HSV in serum, showing a sensitivity of 93% and a specificity of 100% for anti-HSV IgG, and a sensitivity of 92% and a specificity of 93% for anti-HSV IgM, according to the manufacturer. However, they do not distinguish between HSV-1 and HSV-2.
Blood markers of neurodegeneration
Serum NfL and plasma pTau-181 levels were measured on the ultra-sensitive single-molecule array (Simoa®) SR-X platform (Quanterix, MA, USA) following the manufacturer’s instructions. The Simoa® NF-light Advantage v2 (Cat. Nº 104,073) and pTau-181 Advantage v2.1 (Cat. Nº 104,111) assays were purchased from Quanterix. These assays measure serum NfL and plasma pTau-181 levels with a detection limit of 0.141 and 1.04 pg/ml, respectively. Two quality control samples were run on each plate for each analyte. Determinations were run in duplicates, and the average of the two measurements (pg/ml) was used for statistical analysis. Samples with coefficients of variation above 20% were repeated.
APOE genotyping
Genomic DNA was isolated from blood using a standard salting-out protocol [
26]. APOE polymorphisms were determined with pre-designed TaqMan® single nucleotide polymorphism genotyping assays (Applied Biosystem™, Thermo Fisher Scientific).
Acquisition and preprocessing of amyloid PET data
Participants were injected with 300 MBq of 18 F-Florbetaben (FBB, NeuraCeq™, Curium Pharma) 90 min before image acquisition in a Philips Gemini 16 PET/CT scanner (Philips, Best, Netherlands). They underwent a 20-min FBB-PET scan in dynamic mode consisting of four frames of 5 min each. Each frame was inspected for excessive motion. As no excessive head motion was detected in FBB images, the four frames were averaged to create a single static FBB image used for quantitative analysis. Amyloid PET scans were corrected for radioactive decay, dead time, attenuation, and scatter, and they were reconstructed iteratively with an isotropic voxel resolution of 2 mm3.
Partial volume correction (PVC) of FBB images was performed with PetSurfer (
https://surfer.nmr.mgh.harvard.edu/fswiki/PetSurfer) using the geometric transfer matrix-derived region-based voxel-wise method and assuming a uniform 6 mm point spread function [
27]. Briefly, FBB images were first co-registered to T1 scans. Next, PVC-cortical FBB images were transformed into standardized uptake value ratio (SUVR) using the mean PVC uptake of the cerebellar GM as reference region. Resulting PVC-FBB cortical-to-cerebellum SUVR images were mapped into individual cortical surfaces and they were finally smoothed with non-linear spherical wavelet-based de-noising schemes [
28].
Acquisition and preprocessing of MRI data
Brain images were acquired on a 3T Philips Ingenia MRI scanner using a 32-channel receive-only radio-frequency (RF) head coil and a transmit RF body coil (Philips, Best, Netherlands). The following MRI sequences were acquired in the same session: (i) 3D T1-weighted (T1w) magnetization prepared rapid gradient echo (MPRAGE) in the sagittal plane: repetition time (TR)/echo time (TE) = 2600 ms/4.7 ms, flip angle (FA) = 9°, acquisition matrix = 384 × 384, voxel resolution in acquisition = 0.65 mm3 isotropic, resulting in 282 slices without gap between adjacent slices; and (ii) T2w Fast Field Echo images using a blood-oxygen-level-dependent (BOLD) sensitive single-shot echo-planar imaging (EPI) sequence in the axial plane: TR/TE: 2000 ms/30 ms, FA = 80°, acquisition matrix = 80 × 80 mm, voxel resolution in acquisition = 3 mm3 isotropic, resulting in 35 slices acquired in posterior to anterior phase-encoding direction with 1 mm of gap between adjacent slices. Pulse and respiratory rates were simultaneously recorded using the scanner’s built-in pulse oximeter placed on the left-hand index finger and a pneumatic respiratory belt strapped around the upper abdomen, respectively. Before starting the acquisition of the EPI sequence, participants were asked to remain still and keep their eyes closed without falling sleep. We acquired 250 EPI scans preceded by 4 dummy volumes to allow time for equilibrium in the spin excitation. To facilitate optimal B1 shimming, a B1 calibration scan was applied before starting the EPI sequence. Brain images were visually examined after each MRI sequence; they were repeated if evident artifacts were identified. All participants underwent the same MRI protocol in the same scanner at the research MRI facility of Pablo de Olavide University.
T1w scans were preprocessed using Freesurfer v6.0 (
https://surfer.nmr.mgh.harvard.edu/). The Freesurfer’s pipeline included brain extraction, automated tissue segmentation, generation of white matter (WM) and pial surfaces, correction of surface topology and inflation, co-registration, and projection of cortical surfaces to a sphere for the purpose of establishing a surface-based coordinate system [
29]. Pial surface misplacements and erroneous WM segmentation were manually corrected on a slice-by-slice basis by one experienced technician. All processing steps were visually checked for quality assurance.
The resting state-fMRI (rs-fMRI) data were preprocessed using AFNI functions (
https://afni.nimh.nih.gov/afni), version AFNI_20.3.01. For each participant, high-frequency spikes were eliminated (
3dDespike), time-locked cardiac (measured by pulse oximeter) and respiratory motion artifacts on brain BOLD signals were minimized using RETROICOR [
30], time differences in slice-acquisition were corrected (
3dTshift), EPI scans were aligned using the first volume as reference and rigid body motion correction (
3dVolreg), and aligned EPI scans were co-registered to their corresponding T1w volumes (
align_epi_anat.py; cost function: lpc + ZZ). Dynamics were removed provided that more than 5% of voxels exhibited signal intensities that deviated from the median absolute deviation of time series (
3dToutcount), and/or when the Euclidean norm (
enorm) threshold exceeded 0.3 mm in head motion. None of the participants showed more than 20% of artifactual dynamics after applying censoring. Simultaneous regression was further applied to minimize the impact of non-neuronal fluctuations on the rs-fMRI signal (
3dTproject). Nuisance regressors included: (i) six head motion parameters (3 translational and 3 rotational) along with their first-order derivatives, (ii) time series of mean total WM/CSF signal intensity, and (iii) cardiac and respiratory fluctuations plus their derivatives to mitigate effects of extracerebral physiological artifacts on brain BOLD signals.
Preprocessed rs-fMRI scans were projected onto the 5th order icosahedral tesselation of the average cortical surface. Seeds for rs-FC analyses were obtained from cortical regions showing significant correlations between anti-HSV IgG levels and FBB binding. Surface-based rs-FC seed to whole cortex maps were computed using the Fisher’s z-transform of the corresponding Pearson’s correlation coefficients.
Statistical analysis
One-way ANOVAs were performed to analyze anti-HSV IgG differences as a function of sex or APOE4 genotype. Multiple linear regression analyses were further performed to evaluate associations of anti-HSV IgG levels with age, NfL and pTau-181, separately. These analyses were adjusted by age, sex, and APOE4 as long as they were not regressors of interest.
We next assessed associations between anti-HSV IgG levels and cognitive function. Five cognitive domains were analyzed: memory, language, attention, processing speed, and executive function. To improve normality and alleviate heteroscedasticity, composite Z-scores for each cognitive domain were Yeo-Johnson transformed [
31]. A four-step mixed effects model including random intercepts across participants was applied to reduce de variance of fixed effect estimates [
32]. The covariates were included in the first step, whereas the main effects (i.e., cognitive domain, anti-HSV IgG levels, anti-HSV IgG-related variations in cortical FBB binding, age, sex, and APOE4) as well as the two- three- and four-way interactions were sequentially added in subsequent steps. We finally applied ANOVAs to compare the different models. These analyses were performed with R Statistical Software v3.0.1 (R Foundation for Statistical Computing, Vienna, Austria).
Vertex-wise multiple linear regression analysis was conducted on cortical surfaces to assess associations between anti-HSV IgG levels and variations in FBB binding. FBB cortical maps and anti-HSV IgG levels were previously Box-Cox transformed to improve normality and alleviate heteroscedasticity [
33]. Significant cortical regions resulting from this analysis were used as seeds to evaluate whether positive rs-FC patterns were indeed moderated by anti-HSV IgG levels. All statistical models were adjusted for age, sex, and APOE4. We next assessed whether APOE4 moderated associations between anti-HSV IgG levels and cortical FBB binding. This statistical model was adjusted for age and sex.
Cortical surface-based analyses were performed using the SurfStat package (
https://www.math.mcgill.ca/keith/surfstat/). Results were corrected for multiple comparisons using a validated hierarchical statistical model [
34]. This procedure first controls the family-wise error rate in significant clusters over smoothed statistical maps applying random field theory; and it next controls the false discovery rate in vertices of significant clusters over unsmoothed statistical maps. The anatomical location of clusters that survived corrections was identified by the location of each cluster’s peak vertex on the Desikan-Killiany atlas [
35]. After the inferential evidence of a significant effect, the standardized local effect size (f
2local or rho
local) was further computed within the context of a multivariate regression model [
36]. To establish the precision of effect sizes, 95% confidence intervals (CI
95%) were estimated with the function
bootci implemented in Matlab (10.000 bootstrap samples), using the normal approximated interval with bootstrapped bias and standard error.
Bayesian linear regression analysis was applied to peak vertices of significant cortical cluster using JASP, version 0.12.2 (
https://jasp-stats.org/). The Bayesian approach allowed us, on the one hand, to interpret results in terms of the magnitude of evidence in favor of the alternative hypothesis as opposed to the null hypothesis and, on the other, to overcome the problem of multiple comparisons across statistical contrasts. Bayesian linear regression analyses were based on the Jeffreys-Zellner-Siow prior using an r scale of 0.354 [
37]. The strength of the Bayes factor for the model including all covariates of no interest (null model) was compared with the model including the predictor of interest (experimental model) (BF
10). BF
10 values were interpreted using the classification scheme proposed by Lee and Wagenmakers [
38]. We only reported results that met all the following criteria: (i) the
p value yielded by the frequentist approach was < 0.05 after correction for multiple comparisons, (ii) the standardized local effect size was at least moderate and the CI
95% did not contain zero, and (iii) the evidence in favor of the alternative hypothesis was at least moderate (BF
10 ≥ 3).
Discussion
Growing evidence suggests that HSV-1 is involved in the pathogenesis of AD, probably contributing to neuroinflammation decades before the onset of clinical symptoms. Despite this, no study to date has provided human in vivo evidence linking HSV infection to AD pathology in normal aging, a phase of life in which HSV reactivation in the brain increases in parallel with a decrease in adaptive immunity and a higher permeability of the blood-brain barrier (BBB). In line with our initial hypothesis, we showed a positive association between anti-HSV IgG levels and Aβ load in fronto-temporal regions of cognitively normal older adults. Interestingly, cortical regions affected by this relationship also exhibited abnormal patterns of resting-state hemodynamic synchronization with the precuneus and temporal lobe, two cortical regions largely affected by AD pathology from the earliest stages. We further found that associations between HSV infection and Aβ load, particularly in the anterior cingulate cortex, were moderated by the APOE4 genotype. Importantly, anti-HSV IgG levels were unrelated to either subclinical cognitive deficits or to blood markers of neurodegeneration, suggesting that anti-HSV IgG was selectively associated with cortical Aβ aggregation in normal aging. To our knowledge, these results provide the first evidence that HSV infection relates to cerebral Aβ deposition in normal aging, supporting the inclusion of cognitively normal older adults in prospective trials of antimicrobial therapy aimed at decreasing the AD risk in the aging population.
The relationship between aging-related deregulations of the immune response, HSV infection, and cerebral Aβ burden is extremely complex. Each of these elements separately may alter the integrity of the BBB, facilitating the transport of HSV into the brain parenchyma, and ultimately exacerbating Aβ aggregation and neuroinflammation [
39]. Alternatively (or reciprocally), the increased production of pro-inflammatory cytokines and plasma cells with aging [
40,
41] may also lead to an increased production of autoantibodies in body fluids aimed at maintaining adaptive immunity through B cell dysfunction [
42]. From this perspective, increased serum levels of anti-HSV IgG may be interpreted as a mere epiphenomenon of inflammaging, which may contribute to accelerated immunosenescence and HSV reactivation [
43,
44], and incidentally relate to cortical Aβ aggregation in normal aging. While the latter explanation is mechanistically plausible, recent evidence indicates that the seroprevalence of anti-HSV IgG, but not the anti-CMV IgG seroprevalence or anti-CMV IgG levels, doubled the risk of dementia in a cohort of non-demented older adults [
45]. On the basis of these results, it does not appear that the pro-inflammatory cytokines-induced production of autoantibodies alone can explain the association between serum levels of anti-HSV IgG and cortical Aβ load in normal aging.
Mounting data indicates that HSV-1 is the human pathogen most frequently linked to Aβ deposition [
5,
46], but molecular mechanisms instigating this relationship are not well understood. For instance, acute HSV-1 infection has been shown to alter the APP processing [
14] and to increase the enzymes responsible for Aβ formation [
47], leading to Aβ accumulation [
15]. Increasing research has revealed that Aβ peptides may also have antiviral, antimicrobial and pathogen agglutination properties [
48‐
51], likely due to their common mechanisms in membrane-disruption ability, to their significant role in the biofilm formation sequestering and neutralizing the microbes, and to the extended view that microbial agglutination inhibits microbial entry in cells and facilitates microbial clearance [
52]. A chronic activation of these pathways triggered by infections may ultimately exacerbate Aβ deposits, leading to AD progression [
53].
The association between anti-HSV IgG levels and cortical Aβ load was restricted to frontal and temporal regions, which is consistent with post-mortem evidence of HSV-1 DNA in Aβ plaques located in temporal and frontal cortices of AD patients and non-demented aged individuals [
16]. Together, these results suggest that although Aβ aggregation is likely a programmed immune response to microbial pathogens, the excess buildup of toxic Aβ aggregates results in a chronic inflammatory response that causes AD pathology [
5,
54]. Importantly, the relationship between increased risk of dementia and seroprevalence of anti-HSV IgG [
45] reinforces the hypothesis that increased levels of anti-HSV IgG in the periphery play a role in dementia development instead of merely reflecting a general augment of blood cells in response to the higher expression of pro-inflammatory cytokines in aging.
Our study further revealed that individuals showing the highest levels of anti-HSV IgG exhibited abnormal patterns of rs-FC with other cortical regions showing early AD pathology, such as the precuneus and temporal cortices [
55]. Previous research has shown that HSV-1 binding to cell membranes causes abnormal functional responses in cortical neurons [
56,
57]. Thus, HSV-1 infection induced hyperexcitability in cultured rat cortical neurons that increased intracellular Ca2 + signaling, affecting APP processing and ultimately leading to Aβ accumulation [
56]. Additionally, HSV-1 infection markedly reduced the expression of synaptic proteins at presynaptic level and impaired synaptic transmission via GSK-3-dependent intraneuronal accumulation of Aβ, which in turn was instigated by intracellular Ca2 + dyshomeostasis [
57]. We speculate that this cascade of neuronal events may underlie abnormal regional patterns of rs-FC in individuals showing the highest levels of anti-HSV IgG. However, it remains unclear whether these abnormal patterns of rs-FC represent a compensatory brain response aimed at maintaining the cognitive performance in the absence of obvious signs of neurodegeneration. This alternative hypothesis may account for the lack of relationship between HSV infection and cognitive deficits in the present study.
APOE4, the most common genetic risk factor for developing AD, has been associated with increased susceptibility to HSV-1 infection [
20]. Additionally, carriers of the APOE4 genotype who experienced frequent reactivations of HSV-1 had a threefold increased risk of AD [
19,
21]. In line with these findings, the present study revealed a moderating role for the APOE4 in the relationship between anti-HSV IgG levels and Aβ load in the anterior cingulate cortex (ACC). The ACC plays a pivotal role in memory, attention and emotions, and it has been considered an integrative hub for decision-making, socially-driven interactions, and empathy-related responses [
58,
59]. Evidence suggests that ACC hypometabolism not only is most pronounced in AD patients carrying the APOE4 genotype [
60], but it may also predict aging-related cognitive decline [
61]. Importantly, the ACC has further shown to accumulate Aβ pathology from early stages of AD [
62], which together with HSV-1 infection may boost the excitation/inhibition imbalance of neural circuitries in ACC [
63].
This study has some limitations that need to be considered. Although participants were well characterized, the sample size is small and results should be interpreted carefully and replicated with larger cohorts. To minimize this limitation, we only reported results that survived multiple comparisons, showed effect sizes above the minimum established, and were further supported by Bayesian evidence. Moreover, the study sample has twice more females than males, which may impact results given the unquestionable role of gender in the development and outcomes of immune responses [
64,
65]. Indeed, 80% of autoimmune diseases occur in women, who typically show stronger immune responses than males. Stronger responses in women produce faster pathogen clearance and better vaccine responsiveness, but they also contribute to increased susceptibility to inflammatory and auto-immune diseases [
66]. While statistical analyses were adjusted by sex to minimize gender effects on results, the small sample size impeded us to specifically assess whether sex plays a role in the relationship between HSV infection and Aβ load. Future research should clarify whether the results reported in the present study are restricted to women or occur similarly in both sexes. Given the cross-sectional nature of our study, it remains challenging to establish the prognostic value of these results in AD development. Future longitudinal studies should be carried out to determine to what extent serological evaluation of HSV infection predicts AD progression in cognitively normal older adults. Importantly, increased levels of anti-HSV IgG in serum neither guarantees the occurrence of HSV in the brain nor informs on whether the infection is active or latent. In general, HSV IgM positivity in the periphery is considered a marker of active infection and it probably indicates a recent reactivation. Unfortunately, the small number of positive HSV IgM participants in our sample (
N = 5) precluded the possibility to study them as a separate group. Finally, associations of HSV with Aβ pathology and/or interactions with APOE4 may have been influenced by HSV-2 infection. However, the overall seroprevalence of HSV-2 in older adults is about 12% [
67] and anti-HSV-2 IgG levels have not shown significant interactions with APOE4 genotype [
68].
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.