Background
Dental amalgam was considered the most commonly used restorative material owing to its ease of use, high durability, and affordable cost [
1]. However, dental resin composite has been widely used nowadays as a substitute for dental amalgam due to the concerns about the possible hazardous risks on human health through mercury content in the amalgam alloy as well as the unesthetic appearance [
2]. Resin composite restorative materials have gained high acceptance and preponderance as they exhibited higher physical, mechanical, optical, thermal, and esthetic characteristics in addition to non-toxic and antibacterial properties [
3‐
5].
Dental resin composite consists of a continuous phase of an organic resin matrix and dispersed phase of inorganic fillers [
6]. The resin matrix constitutes of monomers, diluents, photoinitiators, accelerators, and coupling agents [
7]. In the 1960s, polymethyl methacrylate (PMMA) was introduced to the dental market with certain limitations such as high shrinkage rate and improper viscosity. Consequently, bisphenol-A glycidyl methacrylate (Bis-GMA) monomer has been used to overcome these drawbacks [
8,
9]. Triethylene glycol dimethacrylate (TEGDMA) and 2-hydroxyethyl methacrylate (HEMA) were utilized as diluents to decrease the viscosity of Bis-GMA for adequate clinical use [
3]. A variety of ceramics, glasses, and silica particles with different sizes and morphologies were employed as fillers for resin composites [
10].
Variable amounts of resin matrices and inorganic fillers are combined to fabricate dental composite formulations [
4]. The organic matrix and the inorganic fillers are both linked together by silane coupling agent which enhances the particle wetting and maximizes the filler loading [
11]. This organic-nonorganic coupling agent is bonded to the filler particles by its nonorganic end and bonds with the resin matrix by its organic end [
12]. A strong bond between the polymer matrix and the filler particles is essential to assure a high level of performance from the composite restoration [
13].
The most substantial developments in resin composite restorative materials were related to the fillers with less attention to improvements in the resinous matrix [
14,
15]. Polymerization shrinkage and its associated stress was considered one of the serious drawbacks of resin composites related to the organic matrix causing loss of marginal integrity, gap formation, micro-leakage, recurrent caries, post restorative sensitivity, and pulpal irritation [
16,
17]. The recent enhancements are focused mainly on the polymeric matrix to provide systems with less polymerization shrinkage. Several low-shrink composites were introduced to the dental markets; Ormocer (organically modified ceramic) is one of these materials which is considered as an alternative for Bis-GMA-based composites [
18,
19]. This material can be properly described as a three-dimensionally cross-linked copolymer that combines organic and inorganic components at a nanoscopic scale through solution and gelation process. The Siloxane oligomeric nanostructure (Si–O–Si) is produced through the hydrolysis and the polycondensation of functionalized alkoxysilanes groups [
20]. Initially, Ormocers were incorporated with conventional methacrylates, but recently, a material formulated with a completely Ormocer-based resin matrix has been developed [
21].
Adequate polymerization is a fundamental factor in achieving optimal mechanical properties and enhanced clinical performance of resin composite materials [
22]. DC affords a qualitative and quantitative indicator for the extent of the polymerization [
23]. DC has a great influence on the resin composites characteristics such as flexural strength, dimensional stability, solubility, and the extent of discoloration and degradation [
24]. Surface hardness also is an important property that enables the resin composite to resist plastic deformation, penetration and scratching which is necessary for esthetic properties and indicates the easiness of finishing and polishing procedures. The microhardness measurement of resin composite can be also a valuable indicator of DC and thus, the clinical success of resin composite restorations [
25,
26].
The temperature of composite restorative materials before curing greatly influences the polymerization process, which in turn affects the properties of the resultant polymer [
27]. A temperature between 4 and 20 °C is usually recommended for adequate composite storage to ensure maximum effectiveness [
28]. Some clinicians store resin composites in the refrigerators at 2–5 °C with the objective of extending their shelf life particularly in hot climate regions [
29]. Moreover, some manufacturers usually recommend keeping the composite syringes inside the refrigerator. However, a debate exists about the effect of storing composite restorative materials in the refrigerator. Therefore, this in-vitro study was conducted to assess the effect of storage temperature on DC and microhardness of three composite restorative materials with different matrix systems.
Discussion
This study was conducted to focus on the impact of refrigeration on composite properties as the refrigerated storage of composite materials is a daily practice. Moreover, there is no sufficient information about the effect of storage temperature on Ormocer-based composites which might be attributed to the recent introduction of the material and lack of conducted studies evaluating this certain point. Therefore, an Ormocer-based and a nanoceramic composites were compared to a conventional nanohybrid based composite. To minimize the variability in the current study, the selected materials where all of approximate filler loading in order to exert more focus on the relation between the evaluated properties and the difference in matrix formulation.
In this in vitro study, the disc-shaped composite specimens were fabricated by specially designed cylindrical plastic mold to prevent stickiness of the composite materials during specimens’ preparations. The thickness of composite specimens was 2-mm to ensure appropriate light penetration and uniform polymerization [
38]. Composite specimens were kept in a dry dark condition for 24 h after light curing before testing to prohibit any light passage to the made samples [
39]. As well, to reach the maximum DC as considerable increase in DC takes place even after the removal of the light curing source, lasting up to 24 h after polymerization [
40]. To control the parameters which may inadvertently affect the final results, all tested specimens were submitted to a standardized fabrication technique, light-curing intensity, light-exposure time, and curing distance between the specimen’s surface and the light curing device’s tip.
DC was evaluated directly using a FTIR-ATR method being a well-accepted and a widely used as it eliminates the need of sample grinding into powder or preparing potassium bromide (KBr) plates which simplifies sample preparation and allows curing under conditions closer to clinical cases. Moreover, this technique permits repeated analyses of the same location on a surface, thus ensuring high comparability between spectra before and after curing [
41]. The area and amplitude ratios between the two main reference peaks (C=C absorption bands at 1607 cm
−1 and 1637 cm
−1) were used to determine the DC of resin composites. The only exception was applied to the Ormocer-based composite; Admira Fusion considering the peak position at 1588 ± 4 cm
−1 as the reference peak [
42].
Vickers microhardness test was used for this study as it is relatively simple, suitable for composite materials due to its higher stability, and reliable for obtaining adequate results [
39]. Vickers microhardness analysis is reported as an indicator for the degree of polymerization of resin composites and used commonly as indirect technique to evaluate DC. Direct evaluation of composites’ DC, when combined with measuring the microhardness as a way to indirectly evaluate the DC has proven to be effective in characterizing the behavior of composite materials in dental practice [
43]. The outcome of this study revealed that the microhardness results were generally correlated with the results of DC, suggesting that the highly polymerized composites with efficient polymeric network and high cross-linking have been shown to exhibit better microhardness. This comes in agreement with previous studies [
44,
45] which showed that there was a correlation between hardness and DC independently of filler type and content. The statistically significant differences in DC and microhardness tests among all tested composite materials at both storage temperatures might be attributed to the difference in the matrix formulation chemistry of these materials which is considered an influential parameter in determining the final outcomes [
46].
Both Ormocer-based and nanocermaic composites share the same Ormocer molecule in their matrix composition leading to a significant higher DC when compared to the nanohybrid composite. Ormocer molecule allows for formation of an inorganic link of siloxane (Si–O–Si) network through hydrolysis and polycondensation reactions. This results in a long inorganic ceramic polysiloxane matrix with lateral organic units, which can react during conventional light-activated polymerization [
47]. The numerous organic polymerizable units for Ormocers might increase the probability of interactions and chemical bonding with neighbor molecules and thus increased the degree of cross-linking and monomer conversion. Whilst, the Bis-GMA molecule in nanohybrid composite has only two polymerizable units [
48]. Conversely, Andrzejewska [
49] reported a reduced double bond conversion for Ormocers. Their explanation was based on the denser network of Ormocer in which the double bonds are less reachable upon polymerization as a result of the steric hindrance of the Ormocer matrix as well as the glass filler particles.
The significant lower DC results for all refrigerator-stored groups when compared to their corresponding room-stored groups may be attributed to the effect of the low temperature in increasing the material’s viscosity. This may have decreased the movement of monomers and retarded the speed of the polymerization reaction. Consequently, a lower extent of the monomer conversion has been achieved [
50]. Daronch et al. [
51] reported that refrigerator-stored composites should not be used clinically until reaching room temperature at least.
Regarding microhardness, the filler particles with natural properties for Ormocer-based composite and nanoceramic composite could have increased the microhardness values via intense ionic inter-atomic bonds, which might explain such insignificantly different results [
52]. However, the significant higher values for these both composites when compared to the nanohybrid composite might be attributed to the reported higher DC in addition to the accompaniment of the high-density organic matrix with hard glass fillers yielding a structure approximately as hard as glass. The spherical morphology of nanoceramic composite’s fillers could be the reason for the increased packing of particles which may have increased the hardness [
53]. The significant decrease in the microhardness values of refrigerator-stored groups could be probably linked to the effect of low temperature which impaired the polymerization quality reflecting low microhardness values. This coincides with Osternack et al. [
54] who reported that refrigerated tested composite materials showed statistically lower hardness values in comparison to their counterparts that polymerized in room temperature. On the contrary, this outcome disagreed with Torres et al. [
55] who reported that composite cooling at 5 °C did not significantly affect the microhardness results.
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