Background
Methods
Study design and subjects
Peripheral blood mononuclear cell isolation
RNA extraction and quantitative real-time polymerase chain reaction (PCR)
Primers | Sequences |
---|---|
Wnt5a | 5ʹ-ACCACATGCAGTACATCGGAG-3ʹ |
5ʹ-GAGGTGTTATCCACAGTGCTG-3ʹ | |
TNF-α | 5ʹ-AGCTGCGCAGAATGAGATGAGTT-3ʹ |
5ʹ-CAGATAGATGGGCTCATACC-3ʹ | |
IL-6 | 5ʹ-ACCCCTGACCCAACCACAAAT-3ʹ |
5ʹ-AGCTGCGCAGAATGAGATGAGTT-3ʹ | |
IL-1β | 5ʹ-AAACAGATGAAGTGCTCCTTCCAGG-3ʹ |
5ʹ-TGGAGAACACCACTTGTTGCTCCA-3ʹ | |
GAPDH | 5ʹ-GCACCGTCAAGGCTGAGAAC-3ʹ |
5ʹ-TGGTGAAGACGCCAGTGGA-3ʹ |
Immunohistochemical staining
Laboratory indices
Statistical analysis
Results
The characteristics of the subjects
Variables | Healthy controls (n = 20) | CHB (n = 82) | ACHBLF (n = 31) |
---|---|---|---|
Age (years) | 41.5 (32.25–46.75) | 38.5 (32–48) | 51 (44–56)△,* |
Sex (male/female) | 13/7 | 52/30 | 22/9 |
ALT (U/L) | 28 (16–34) | 161 (102–308)△ | 188 (64–962)△ |
AST (U/L) | 23.5 (19.3–29.0) | 104.0 (54.0-185.8)△ | 267 (83–913)△,* |
ALB (g/L) | 46.2 (37.9–51.2) | 42.2 (39.5–45.0) | 31.8 (28.4–34.9)△,* |
TBIL (µmol/L) | 10.9 (9.1–17.2) | 15.7 (11.6–23.6) | 166.0 (126.2-220.5)△,* |
HBeAg (+/-) | NA | 58/24 | 20/11 |
Log10 (HBV DNA load) | NA | 7.01 (5.83–7.72) | 6.51 (3.89–7.97) |
PTA (%) | 94.0 (84.5–105.0) | 94.0 (85.0-101.3) | 46.0 (35.0–52.0)△,* |
INR | 1.05 (1.00-1.12) | 1.09 (1.03–1.16) | 1.77 (1.57–2.15)△,* |
mRNA expression of Wnt5a, IL-6, TNF-α and IL-1β in PBMCs from ACHBLF patients, CHB patients, and healthy controls
Correlation between Wnt5a mRNA levels and laboratory indices or inflammatory cytokine expression in CHB patients and ACHBLF patients
Receiver operating characteristic (ROC) analysis of Wnt5a mRNA expression to discriminate ACHBLF from CHB
Association between intrahepatic Wnt5a expression and inflammation in chronic HBV Infection patients
variables | Chronic HBV infection patients (n = 32) | Normal controls (n = 6) | P value |
---|---|---|---|
Age (years) | 39.5 (33-45.75) | 37 (31.25–45.5) | > 0.05 |
Sex (male/female) | 21/11 | 4/2 | > 0.05 |
ALT (U/L) | 26.0 (18.3–33.0) | 21.0 (17.5–26.0) | > 0.05 |
AST (U/L) | 21.5 (16.3–26.0) | 25.0 (18.3–32.5) | > 0.05 |
HBeAg (+/-) | 14/18 | NA | |
Log10 (HBV DNA load) | 5.18 (2.95–7.35) | NA | |
Hepatic inflammatory grade (n) G0/G1/G2/G3/G4 | 0/5/12/14/1 | NA |
Case | Age | Gender | G (Inflammation) | S (Fibrosis) | ALT (U/L) | AST (U/L) |
---|---|---|---|---|---|---|
1 | 55 | Male | 1 | 1 | 33 | 23 |
2 | 47 | Female | 1 | 1 | 18 | 22 |
3 | 41 | Male | 1 | 1 | 31 | 18 |
4 | 47 | Male | 1 | 1 | 21 | 21 |
5 | 43 | Male | 1 | 2 | 13 | 17 |
6 | 33 | Female | 2 | 1 | 5 | 12 |
7 | 38 | Male | 2 | 1 | 27 | 23 |
8 | 53 | Male | 2 | 1 | 20 | 16 |
9 | 26 | Male | 2 | 2 | 20 | 14 |
10 | 36 | Male | 2 | 2 | 19 | 24 |
11 | 35 | Male | 2 | 2 | 21 | 16 |
12 | 34 | Female | 2 | 2 | 21 | 20 |
13 | 45 | Male | 2 | 2 | 32 | 21 |
14 | 32 | Female | 2 | 2 | 20 | 18 |
15 | 45 | Female | 2 | 2 | 50 | 26 |
16 | 49 | Male | 2 | 3 | 96 | 43 |
17 | 40 | Male | 2 | 3 | 32 | 25 |
18 | 25 | Female | 3 | 1 | 32 | 29 |
19 | 33 | Male | 3 | 2 | 79 | 35 |
20 | 58 | Male | 3 | 2 | 25 | 31 |
21 | 48 | Male | 3 | 2 | 16 | 15 |
22 | 39 | Male | 3 | 2 | 29 | 25 |
23 | 31 | Female | 3 | 2 | 16 | 16 |
24 | 46 | Male | 3 | 2 | 37 | 20 |
25 | 34 | Male | 3 | 2 | 78 | 45 |
26 | 32 | Male | 3 | 3 | 32 | 29 |
27 | 41 | Male | 3 | 3 | 45 | 23 |
28 | 34 | Female | 3 | 3 | 11 | 18 |
29 | 26 | Female | 3 | 3 | 33 | 26 |
30 | 43 | Female | 3 | 3 | 37 | 38 |
31 | 31 | Male | 3 | 4 | 16 | 10 |
32 | 45 | Female | 4 | 2 | 12 | 13 |
Variables | Chronic HBV infection patients with low hepatic inflammatory grade (G0-2) (n = 17) | Chronic HBV infection patients with high hepatic inflammatory grade (G3-4) (n = 15) | P value |
---|---|---|---|
ALT(U/L) | 21.0(19.5–32.0) | 32.0(16.0–37.0) | >0.05 |
AST(U/L) | 21.0(16.5–23.5) | 25.0(31.0–45.0) | >0.05 |