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Histocompatibility and Donor Selection

Influence of mannose-binding lectin genotypes and serostatus in allo-SCT: analysis of 131 recipients and donors

Bone Marrow Transplantation volume 45, pages 13–19 (2010)Cite this article

Abstract

Mannan-binding lectin (MBL) deficiency is determined by MBL gene polymorphisms and is associated with an increased infection risk. To clarify the role of MBL in Allo-SCT, 131 recipients–donors were analysed. MBL genotypes were determined by PCR and heteroduplex analyses, MBL serum levels by ELISA, and MBL oligomers by western blotting. MBL levels <400 ng/ml were associated with increased susceptibility to fungal pneumonia (7/12 vs 35/111; P=0.04, adjusted P=0.002), HSV/VZV (7/12 vs 26/111; P=0.03), CMV reactivation and acute GVHD. Donor genotypes had no influence. Pre-SCT MBL levels corresponded to recipients’ genotypes (P<0.001), changed significantly post-SCT, but were not influenced by donors’ genotypes. MBL oligomer profiles were similar pre-/post-SCT. Cultured CD34+ cells were found not to synthesise MBL. In conclusion, low MBL levels pre-transplant predisposed patients to sepsis, fungal and viral infection. Donors’ MBL genotypes did not influence infection rates. Prospective studies should clarify the importance of MBL as a prelude for MBL replacement after SCT.

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Acknowledgements

We thank the staff of the Infectious Diseases and Microbiology Unit for assistance with the MBL assays. RT was supported by a grant from the Agence nationale de recherches sur le sida et les hépatites virales (ANRS).

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Authors and Affiliations

  1. Infectious Diseases and Microbiology Unit, Institute of Child Health and Great Ormond Street Hospital, London, UK

    O W Neth, R Thiébaut, R M Haston & N Klein

  2. Department of Paediatric Infectious Diseases and Immunology, Hospital Universitario Virgen del Rocio, Sevilla, Spain

    O W Neth

  3. Department of Stem Cell Transplantation, University Cancer Center Hamburg, Hamburg, Germany

    U Bacher, P Das, T Zabelina, H Kabisch, N Kroeger, F Ayuk, M Lioznov, O Waschke, B Fehse & A R Zander

  4. Department of Pediatric Hematology and Oncology, University of Hamburg, Hamburg, Germany

    H Kabisch

  5. Department of Experimental Pediatric Oncology and Hematology, University Hospital of the Johann Wolfgang Goethe-University, Frankfurt am Main, Germany

    B Fehse

  6. INSERM U897 Epidemiology and Biostatistics, Bordeaux 2 University, Bordeaux, France

    R Thiébaut

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  1. O W Neth
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Correspondence to O W Neth.

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Neth, O., Bacher, U., Das, P. et al. Influence of mannose-binding lectin genotypes and serostatus in allo-SCT: analysis of 131 recipients and donors. Bone Marrow Transplant 45, 13–19 (2010). https://doi.org/10.1038/bmt.2009.90

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  • DOI: https://doi.org/10.1038/bmt.2009.90

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