Introduction
Materials and methods
Study design and animals
Study design
Histological processing
Histological and histomorphometrical analysis
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The line connecting the bone crest at the oral and vestibular aspects (BC);
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The horizonal line (H) dividing the tooth root into the coronal (C) and apical (A) sections;
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The PLS marking a space between the tooth and surrounding alveolar bone;
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Rectangles of 0.6 mm2 depicting the alveolar bone surrounding the tooth root in sections C and A and the apex (Ap).
Statistical analysis
Results
Phase 1 (17 weeks; n = 21 animals) | |||||||
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Group | PLS-C (mm) | PLS-A (mm) | EL-C (No.) | EL-A (No.) | EL-Ap (No.) | ICI (mm2) | MT (mm) |
Zo (n = 5) | n = 1 | ||||||
Mean SD 95% CI Min Max | 0.17 0.1 (0.08, 0.26) 0.06 0.32 | 0.15 0.08 (0.09, 0.22) 0.06 0.27 | 5.9 2.33 (3.86, 7.94) 2.5 9 | 6.5 2.87 (3.98, 9.02) 2 9 | 2.3 3.19 (-0.5, 5.1) 0 6.5 | 0.9 - - 0.9 0.9 | 0.26 0.02 (0.24, 0.28) 0.23 0.29 |
De (n = 3) | n = 0 | ||||||
Mean SD 95% CI Min Max | 0.14 0.02 (0.12, 0.16) 0.13 0.16 | 0.14 0.02 (0.12, 0.16) 0.13 0.16 | 2.83 0.76 (1.97, 3.7) 2 3.5 | 2.08 1.38 (0.53, 3.64) 0.75 3.5 | 4 3.46 (0.08, 7.92) 0 6 | - | 0.26 0.07 (0.19, 0.34) 0.19 0.32 |
Be (n = 3) | n = 1 | ||||||
Mean SD 95% CI Min Max | 0.31 0.16 (0.13, 0.48) 0.18 0.48 | 0.24 0.18 (0.04, 0.44) 0.14 0.44 | 6.5 4.09 (1.87, 11.13) 3 11 | 2.67 2.08 (0.31, 5.02) 1 5 | 1 1 ( -0.13, 2.13) 0 2 | 0.1 - - 0.1 0.1 | 0.36 0.15 (0.19, 0.53) 0.27 0.54 |
Zo + Be (n = 2) | n = 2 | ||||||
Mean SD 95% CI Min Max | 0.15 0.02 (0.12, 0.17) 0.13 0.16 | 0.15 0.04 (0.09, 0.21) 0.12 0.18 | 5.25 1.77 (2.8, 7.7) 4 6.5 | 5.25 1.06 (3.78, 6.72) 4.5 6 | 6.75 0.35 (6.26, 7.24) 6.5 7 | 0.38 - - 0.38 0.38 | 0.26 0.06 (0.18, 0.35) 0.22 0.31 |
De + Be (n = 3) | n = 0 | ||||||
Mean SD 95% CI Min Max | 0.23 0.06 (0.16, 0.3) 0.16 0.26 | 0.19 0.03 (0.16, 0.23) 0.17 0.23 | 3.83 1.76 (1.85, 5.82) 2 5.5 | 5.67 2.08 (3.31, 8.02) 4 8 | 3.67 3.21 (0.03, 7.3) 0 6 | - | 0.22 0.06 (0.15, 0.28) 0.18 0.28 |
Control (n = 5) | n = 1 | ||||||
Mean SD 95% CI Min Max | 0.21 0.11 (0.11, 0.31) 0.09 0.37 | 0.15 0.04 (0.11, 0.19) 0.12 0.23 | 8.8 4.86 (4.54, 13.06) 4.5 16.5 | 4.1 2.38 (2.01, 6.19) 1.5 7.5 | 3.2 3.35 (0.27, 6.13) 1 9 | 0.1 - - 0.1 0.1 | 0.29 0.04 (0.25, 0.32) 0.22 0.33 |
Phase 2 (29 weeks; n = 34 animals) | |||||||
Group | PLS-C (mm) | PLS-A (mm) | EL-C (No.) | EL-A (No.) | EL-Ap (No.) | IICI (mm2) | MT(mm) |
Zo (n = 2) | n = 0 | ||||||
Mean SD 95% CI Min Max | 0.16 0.02 (0.12, 0.19) 0.14 0.17 | 0.12 0.03 (0.08, 0.17) 0.1 0.15 | 12.5 2.12 (9.56, 15.44) 11 14 | 9.75 4.6 (3.38, 16.12) 6.5 13 | 3.75 2.47 (0.32, 7.18) 2 5.5 | - | 0.17 0.07 (0.08, 0.27) 0.13 0.22 |
De (n = 8) Mean SD 95% CI Min Max | 0.16 0.05 (0.13, 0.19) 0.12 0.27 | 0.19 0.06 (0.15, 0.23) 0.13 0.32 | 13.16 7.14 (8.21, 18.1) 3.5 27.5 | 10.34 3.87 (7.66, 13.02) 6 15.5 | 6.69 3.46 (4.29, 9.09) 1 11 | n = 4 0.05 0.03 (0.03,0.07) 0.02 0.1 | 0.21 0.09 (0.15, 0.27) 0.11 0.41 |
Be (n = 7) | n = 5 | ||||||
Mean SD 95% CI Min Max | 0.18 0.05 (0.14, 0.22) 0.1 0.24 | 0.19 0.08 (0.13, 0.25) 0.09 0.33 | 9.75 7.94 (3.87, 15.63) 2.5 26 | 9.86 5.35 (5.9, 13.82) 3 17.25 | 4.86 2.93 (2.69, 7.02) 2 10.75 | 0.12 0.09 (0.06, 0.19) 0.03 0.26 | 0.22 0.09 (0.15, 0.28) 0.13 0.38 |
Zo + Be (n = 4) | n = 2 | ||||||
Mean SD 95% CI Min Max | 0.23 0.07 (0.16, 0.29) 0.17 0.31 | 0.21 0.05 (0.16, 0.26) 0.15 0.26 | 13.94 4.48 (9.55, 18.33) 10.75 20.25 | 9.12 5.65 (3.59, 14.66) 5.5 17.5 | 6.62 3.12 (3.57, 9.68) 2.5 9.5 | 1.1 0.36 (0.74, 1.45) 0.84 1.35 | 0.28 0.06 (0.22, 0.34) 0.22 0.33 |
De + Be (n = 6) | n = 3 | ||||||
Mean SD 95% CI Min Max | 0.16 0.11 (0.07, 0.24) 0.08 0.37 | 0.19 0.09 (0.12, 0.26) 0.13 0.36 | 9.92 5.34 (5.64, 14.19) 3 15.5 | 9.92 3.11 (7.43, 12.4) 7 15.5 | 4.27 4.02 (1.05, 7.49) 0.5 10 | 0.04 0.02 (0.02, 0.06) 0.03 0.07 | 0.22 0.04 (0.18, 0.25) 0.17 0.28 |
Control (n = 7) | n = 2 | ||||||
Mean SD 95% CI Min Max | 0.15 0.04 (0.12, 0.18) 0.11 0.21 | 0.14 0.03 (0.12, 0.16) 0.11 0.19 | 11.29 4.06 (8.28, 14.29) 4.5 17.5 | 10.82 5.53 (6.73, 14.92) 3.75 18.5 | 7.25 2.72 (5.24, 9.26) 3.25 12 | 0.02 0 (0.02, 0.02) 0.02 0.02 | 0.22 0.06 (0.17, 0.26) 0.17 0.34 |